Increased osteoblast functions on nanophase titania dispersed in poly-lactic-co-glycolic acid composites
Increased osteoblast functions on nanophase titania dispersed in poly-lactic-co-glycolic acid composites
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DOI：
10.1088/0957-4484/16/7/038
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发表时间：
2005-07-01
期刊：
影响因子：
3.5
通讯作者：
Webster, TJ
中科院分区：
文献类型：
--
作者：
Liu, HN;Slamovich, EB;Webster, TJ
纳米二氧化钛/聚乳酸-乙醇酸(PLGA)复合材料的设计提供了一种激动人心的方法，将可降解聚合物的优点与纳米陶瓷颗粒相结合，以优化骨再生的物理和生物性能。重要的是，纳米二氧化钛模拟了骨组成成分的尺寸尺度，因为它是由纳米尺寸的羟基磷灰石均匀分散在以胶原为主的基质中的纳米结构复合材料。基于这些原因，本体外研究的目的是研究纳米二氧化钛/PLGA复合材料上成骨细胞(骨形成细胞)的粘附性和长期功能。由于纳米二氧化钛在添加到聚合物中时容易明显团聚，因此在本研究中采用不同的超声输出功率来改善二氧化钛的分散。结果表明，随着超声强度的增加，二氧化钛在PLGA中的分散度增加，成骨细胞的粘附力增强，纳米二氧化钛在PLGA中的分散度提高。此外，与普通PLGA相比，纳米二氧化钛/PLGA复合材料具有更好的成骨细胞长期功能，如碱性磷酸酶活性和含钙矿物沉积。事实上，成骨细胞在以最高功率在PLGA中超声处理的纳米二氧化钛上培养时，产生了最大的胶原蛋白。通过这种方式，本研究表明，具有良好分散的纳米二氧化钛的PLGA复合材料可以增强骨组织工程应用所需的成骨细胞功能。
The design of nanophase titania/poly-lactic-co-glycolic acid (PLGA) composites offers an exciting approach to combine the advantages of a degradable polymer with nano-size ceramic grains to optimize physical and biological properties for bone regeneration. Importantly, nanophase titania mimics the size scale of constituent components of bone since it is a nanostructured composite composed of nanometre dimensioned hydroxyapatite well dispersed in a mostly collagen matrix. For these reasons, the objective of the present in vitro study was to investigate osteoblast (bone-forming cell) adhesion and long-term functions on nanophase titania/PLGA composites. Since nanophase titania tended to significantly agglomerate when added to polymers, different sonication output powers were applied in this study to improve titania dispersion. Results demonstrated that the dispersion of titania in PLGA was enhanced by increasing the intensity of sonication and that greater osteoblast adhesion correlated with improved nanophase titania dispersion in PLGA. Moreover, results correlated better osteoblast long-term functions, such as alkaline phosphatase activity and calcium-containing mineral deposition, on nanophase titania/PLGA composites compared to plain PLGA. In fact, the greatest collagen production by osteoblasts occurred when cultured on nanophase titania sonicated in PLGA at the highest powers. In this manner, the present study demonstrates that PLGA composites with well dispersed nanophase titania can enhance osteoblast functions necessary for improved bone tissue engineering applications.