Whole-genome resequencing reveals loci under selection during silkworm improvement
Whole-genome resequencing reveals loci under selection during silkworm improvement
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全基因组重测序揭示了家蚕改良过程中受到选择的基因座。
DOI：
10.1111/jbg.12513
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发表时间：
2020-10-12
影响因子：
2.6
通讯作者：
Dai, Fangyin
中科院分区：
文献类型：
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作者：
Li, Chunlin;Zuo, Weidong;Dai, Fangyin
育种或遗传改良是指驯化后的人工选择过程；因此，它对现代农业和动物生产产生了重大影响。改进通常集中在对经济表现有很大影响的特征上。因此，了解改良的遗传基础将有助于鉴定控制经济性状的基因，并将促进未来的作物和动物育种。然而，对改善的分子基础的全基因组研究仍然很少。蚕是一种独特的、完全驯化的经济上重要的无脊椎动物；遗传改良对蚕的丝相关性状产生了巨大影响。在此，我们对本地和遗传改良的家蚕品系进行了全基因组测序，以确定在家蚕育种/改良中具有强选择的基因组区域。通过全基因组选择性扫描分析，我们确定了24个具有强选择信号的基因组区域，其中8个区域包含13个候选基因。有趣的是，其中6个基因被标注了与神经信号反应相关的功能。在这6个基因中，bgibmga004050编码蚕creb -regulated_transcription_coactivator_1(BmCRTC1)，据报道该基因参与能量感应通路。这些结果表明，这种改善可能影响了家蚕的神经系统。这项研究将为家蚕和其他物种遗传改良的遗传基础提供新的见解。
Breeding or genetic improvement refers to the process of artificial selection following domestication; as such, it has had a major influence on modern agriculture and animal production. Improvement generally focuses on traits that greatly affect the economic performance. Therefore, understanding the genetic basis underlying improvement will contribute to the identification of genes controlling economic traits and will facilitate future crop and animal breeding. However, genome-wide study of the molecular basis underlying improvement remains rare. The silkworm is a unique, entirely domesticated economically important invertebrate; genetic improvement has had a huge effect on the silkworm regarding silk-related traits. Herein, we performed whole-genomic sequencing on local and genetically improved silkworm lines to identify the genomic regions under strong selection in silkworm breeding/improvement. By genomic-wide selective sweeping analysis, we identified 24 genomic regions with strong selection signals, eight of which contained 13 candidate genes underlying silkworm breeding. Interestingly, six of these genes were annotated with functions related to neural signal response. Among the six genes,BGIBMGA004050encodes silkwormCREB-regulated_transcription_coactivator_1(BmCRTC1), which was reported to be involved in energy-sensing pathways. These results suggested that improvement may have affected the nervous system of the silkworm. This research will provide new insights into the genetic basis underlying the genetic improvement of silkworms and possibly of other species.