Gram-negative pathogen Klebsiella oxytoca is associated with spontaneous chronic otitis media in Toll-like receptor 4-deficient C3H/HeJ mice
Gram-negative pathogen Klebsiella oxytoca is associated with spontaneous chronic otitis media in Toll-like receptor 4-deficient C3H/HeJ mice
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DOI：
10.1080/00016480701387124
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发表时间：
2008-01-01
影响因子：
1.4
通讯作者：
Trune, Dennis R.
中科院分区：
文献类型：
--
作者：
Macarthur, Carol J.;Pillers, De-Ann M.;Trune, Dennis R.
结论本报告通过培养、聚合酶链反应（PCR）和电子显微镜证实了C3 H/HeJ小鼠中耳中存在革兰氏阴性克雷伯菌。细菌病原体支持物的鉴定证实C3 H/HeJ小鼠是自发性慢性中耳炎及其对中耳和内耳影响的良好模型。目标. C3 H/HeJ小鼠在其Toll样受体4中具有单个氨基酸取代，使其对内毒素不敏感并抑制先天免疫系统的启动。本研究通过培养、PCR、组织学和电镜等方法对中耳感染的细菌学进行了探讨。材料与方法。10个月大的C3 H/HeJ小鼠被筛选为自发性中耳炎阳性。在镇静状态下进行小鼠鼓室穿刺和血培养。然后将中耳抽吸物材料和血液样品送去进行培养和PCR。然后处死小鼠进行明视野和电子显微镜分析。结果所有鼓室穿刺和血液标本生长革兰氏阴性产酸克雷伯菌，这是由PCR证实。组织病理学证实中耳和内耳有强烈的炎症反应和革兰氏阴性菌。中耳的电子显微镜检查显示大量杆状克雷伯氏菌，无论是游离的还是被中性粒细胞吞噬的。
Conclusion. This report confirms the presence of This report confirms the presence of ggram-negative Klebsiella bacteria in the middle ear of the C3H/HeJ mouse by culture, polymerase chain reaction (PCR), and electron microscopy. Identification of the bacterial pathogen supportsconfirms that the C3H/HeJ mouse ais an excellent model for spontaneous chronic otitis media and its effects on the middle and inner ear. Objectives. The C3H/HeJ mouse has a single amino acid substitution in its Toll-like receptor 4, making it insensitive to endotoxin and suppressing initiation of the innate immune system. This study explored the bacteriology of the resultant middle ear infection by culture, PCR, histology, and electron microscopy. Materials and methods. Twelve-month-old C3H/HeJ mice were screened positive for spontaneous otitis media. Tympanocentesis and blood cultures of mice were carried out under sedation. Middle ear aspirate material and blood samples were then sent for culture and PCR. Mice were then sacrificed for bright-field and electron microscopy analysis. Results. All tympanocentesis and blood specimens grew gram-negative Klebsiella oxytoca, which was confirmed by PCR. Histopathology confirmed an intense inflammatory reaction and gram-negative bacteria in the middle and inner ears. Electron microscopy of the middle ears revealed abundant rod-shaped Klebsiella bacteria, both free and being engulfed by neutrophils.