Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: Scaffold role of Pbs2p MAPKK
Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: Scaffold role of Pbs2p MAPKK
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DOI：
10.1126/science.276.5319.1702
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发表时间：
1997-06-13
期刊：
影响因子：
56.9
通讯作者：
Saito, H
中科院分区：
文献类型：
--
作者：
Posas, F;Saito, H
酿酒酵母暴露在高渗透压下，诱导Sln1p-Ypd1p-Ssk1p双组分渗透传感器激活由Ssk2p和Ssk22p MAPKKs、Pbs2p MAPKK和Hog1p MAPK组成的丝裂原激活蛋白(MAP)级联。第二个渗透传感器Sho1p也激活了Pbs2p和Hog1p，但通过Ste11p MAPKKK做到了这一点。尽管Ste11p也参与了交配信息素反应MAPK级联反应，但这两条途径之间没有可检测到的串扰。与Sho1p渗透传感器、MAPKKK Ste11p和MAPK Hog1p结合的MAPKK Pbs2p。因此，Pbs2p可能是一种支架蛋白。
Exposure of the yeast Saccharomyces cerevisiae to high extracellular osmolarity induces the Sln1p-Ypd1p-Ssk1p two-component osmosensor to activate a mitogen-activated protein (MAP) kinase cascade composed of the Ssk2p and Ssk22p MAP kinase kinase kinases (MAPKKKs), the Pbs2p MAPKK, and the Hog1p MAPK. A second osmosensor, Sho1p, also activated Pbs2p and Hog1p, but did so through the Ste11p MAPKKK. Although Ste11p also participates in the mating pheromone-responsive MAPK cascade, there was no detectable cross talk between these two pathways. The MAPKK Pbs2p bound to the Sho1p osmosensor, the MAPKKK Ste11p, and the MAPK Hog1p. Thus, Pbs2p may serve as a scaffold protein.