Induction of Apoptosis in Colon Cancer Cells Treated with Isorhamnetin Glycosides from Opuntia Ficus-indica Pads
Induction of Apoptosis in Colon Cancer Cells Treated with Isorhamnetin Glycosides from Opuntia Ficus-indica Pads
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DOI：
10.1007/s11130-014-0438-5
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发表时间：
2014-12-01
影响因子：
4
通讯作者：
Serna-Saldivar, Sergio O.
中科院分区：
文献类型：
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作者：
Antunes-Ricardo, Marilena;Moreno-Garcia, Beatriz E.;Serna-Saldivar, Sergio O.
(OFI)含有促进健康的化合物，如类黄酮，是异鼠李素苷最丰富。我们评估了具有不同异鼠李素糖苷的OFI提取物对两种不同的人结肠癌细胞（HT-29和Caco 2）的作用。提取物，通过碱水解与NaOH在40 A度C，在15，30或60分钟。三和二糖苷是最丰富的异鼠李素糖苷，因此这些化合物被分离出来，以比较其细胞毒性效应与提取物中获得。OFI提取物和纯化的异鼠李素苷对HT-29细胞的细胞毒性比Caco 2细胞更强。OFI-30对HT-29（4.9 A +/- 0.5 μ g/mL）和Caco 2（8.2 A +/- 0.3 μ g/mL）表现出最低的IC 50值。在HT-29细胞中测试时，异鼠李素二苷IG 5和IG 6比纯异鼠李素苷元或三苷的细胞毒性更大。生物发光分析显示，在OFI提取物处理的细胞中，caspase 3/7的活性增加，特别是对于具有最高浓度的异鼠李素三糖苷的提取物。流式细胞术分析证实，OFI提取物和异鼠李素苷诱导HT-29细胞凋亡的百分比高于Caco 2细胞，而异鼠李素在Caco 2细胞中的凋亡更多。这项研究表明，糖基化影响纯异鼠李素糖苷的抗增殖作用，或当它们与其他植物化学物质混合在从OFI获得的提取物。
(OFI) contains health-promoting compounds like flavonoids, being the isorhamnetin glycosides the most abundant. We evaluated the effect of OFI extracts with different isorhamnetin glycosides against two different human colon cancer cells (HT-29 and Caco2). The extracts were obtained by alkaline hydrolysis with NaOH at 40 A degrees C during 15, 30 or 60 min. Tri and diglycosides were the most abundant isorhamnetin glycosides, therefore these compounds were isolated to compare their cytotoxic effect with the obtained from the extracts. The OFI extracts and purified isorhamnetin glycosides were more cytotoxic against HT-29 cells than Caco2 cells. OFI-30 exhibited the lowest IC50 value against HT-29 (4.9 A +/- 0.5 mu g/mL) and against Caco2 (8.2 A +/- 0.3 mu g/mL). Isorhamnetin diglycosides IG5 and IG6 were more cytotoxic than pure isorhamnetin aglycone or triglycosides when they were tested in HT-29 cells. Bioluminescent analysis revealed increased activity of caspase 3/7 in OFI extracts-treated cells, particularly for the extract with the highest concentration of isorhamnetin triglycosides. Flow cytometry analysis confirmed that OFI extract and isorhamnetin glycosides induced a higher percentage of apoptosis in HT-29 than in Caco2, while isorhamnetin was more apoptotic in Caco2. This research demonstrated that glycosilation affected antiproliferative effect of pure isorhamnetin glycosides or when they are mixed with other phytochemicals in an extract obtained from OFI.