Leifsonia psychrotolerans sp. nov., a psychrotolerant species of the family Microbacteriaceae from Livingston Island, Antarctica.
Leifsonia psychrotolerans sp. nov., a psychrotolerant species of the family Microbacteriaceae from Livingston Island, Antarctica.
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DOI：
10.1099/ijs.0.021956-0
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发表时间：
2011-08
影响因子：
2.8
通讯作者：
L. Ganzert;Felizitas Bajerski;K. Mangelsdorf;A. Lipski;D. Wagner
中科院分区：
文献类型：
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作者：
L. Ganzert;Felizitas Bajerski;K. Mangelsdorf;A. Lipski;D. Wagner
从南极洲利文斯顿岛保加利亚圣克莱门特奥赫里德斯基站附近的苔藓覆盖的土壤中分离出一种耐冷、黄色、革兰氏阳性、能运动、兼性厌氧的细菌菌株 LI1(T)。基于比较 16S rRNA 基因序列的系统发育分析将该菌株与 Leifsonia kafniensis KFC-22(T)、Leifsonia pindariensis PON10(T) 和 Leifsonia antarctica SPC-20(T) 划分为一个分支，其序列相似性分别为 99.0%、97.9% 和 97.9%。 DNA-DNA 杂交显示与 L. kafniensis LMG 24362(T) 的重关联值为 2.7%。菌株LI1(T)的DNA G+C含量为64.5mol%。生长温度范围为-6至28°C，最佳生长温度为16°C。生长发生在 0-5% 氯化钠和 pH 4.5-9.5 条件下，最佳生长条件是 1-2% 氯化钠和 pH 5.5-6.5。主要脂肪酸是反异C(15:0)、C(18:0)和异C(15:0)。极性脂质是磷脂酰甘油和二磷脂酰甘油。生理生化测试清楚地将菌株 LI1(T) 与 L. kafniensis 区分开来。因此，提出了 Leifsonia 属内的一种新的耐冷物种：Leifsonia psychrotolerans sp。十一月（类型应变 LI1(T) = DSM 22824(T) = NCCB 100313(T)）。
A cold-tolerant, yellow-pigmented, Gram-positive, motile, facultatively anaerobic bacterial strain, LI1(T), was isolated from a moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St. Kliment Ohridski. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis KFC-22(T), Leifsonia pindariensis PON10(T) and Leifsonia antarctica SPC-20(T), with which it showed sequence similarities of 99.0, 97.9 and 97.9 %, respectively. DNA-DNA hybridization revealed a reassociation value of 2.7 % with L. kafniensis LMG 24362(T). The DNA G+C content of strain LI1(T) was 64.5 mol%. The growth temperature range was -6 to 28 °C, with optimum growth at 16 °C. Growth occurred in 0-5 % NaCl and at pH 4.5-9.5, with optimum growth in 1-2 % NaCl and at pH 5.5-6.5. The predominant fatty acids were anteiso-C(15 : 0), C(18 : 0) and iso-C(15 : 0). The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. Physiological and biochemical tests clearly differentiated strain LI1(T) from L. kafniensis. Therefore, a novel cold-tolerant species within the genus Leifsonia is proposed: Leifsonia psychrotolerans sp. nov. (type strain LI1(T) = DSM 22824(T) = NCCB 100313(T)).