Candida albicans biofilms:: a developmental state associated with specific and stable gene expression patterns
Candida albicans biofilms:: a developmental state associated with specific and stable gene expression patterns
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DOI：
10.1128/ec.3.2.536-545.2004
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发表时间：
2004-04-01
期刊：
影响因子：
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通讯作者：
d'Enfert, C
中科院分区：
文献类型：
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作者：
García-Sánchez, S;Aubert, S;d'Enfert, C
像许多细菌一样，酵母菌可以在几个表面形成生物膜。白色念珠菌在导管、假体和上皮细胞表面定植，形成对抗真菌药物极具抗药性的生物膜。我们已经使用转录图谱来研究白色念珠菌生物被膜的特性。通过整体基因表达相关性，比较了在不同营养条件、好氧条件或葡萄糖浓度条件下产生的生物膜和浮游植物培养物。无论生长条件如何，生物膜之间的相关性都远远高于浮游种群，这表明在不同环境中形成的生物膜种群表现出非常相似和独特的转录谱。鉴定出了第一批325个差异表达基因。与氨基酸生物合成基因在该簇中的过度表达一致，氨基酸代谢调节因子Gcn4P被证明是正常生物膜生长所必需的。为了确定与菌丝发育无关的生物膜相关基因，我们研究了一株野生型菌丝产生菌株和一株cph1/cph1 Efg1/Efg1缺陷菌株所产生的生物膜的转录组。这一分析确定了317个独立于菌丝形成表达的基因簇，而86个基因依赖于菌丝发育。这两组都揭示了硫-氨基酸生物合成途径的激活，这是白色念珠菌生物膜的一个特征。
Like many bacteria, yeast species can form biofilms on several surfaces. Candida albicans colonizes the surfaces of catheters, prostheses, and epithelia, forming biofilms that are extremely resistant to antifungal drugs. We have used transcript profiling to investigate the specific properties of C. albicans biofilms. Biofilm and planktonic cultures produced under different conditions of nutrient How, aerobiosis, or glucose concentration were compared by overall gene expression correlation. Correlation was much higher between biofilms than planktonic populations irrespective of the growth conditions, indicating that biofilm populations formed in different environments display very similar and specific transcript profiles. A first cluster of 325 differentially expressed genes was identified. In agreement with the overrepresentation of amino acid biosynthesis genes in this cluster, Gcn4p, a regulator of amino acid metabolism, was shown to be required for normal biofilm growth. To identify biofilm-related genes that are independent of mycelial development, we studied the transcriptome of biofilms produced by a wild-type, hypha-producing strain and a cph1/cph1 efg1/efg1 strain defective for hypha production. This analysis identified a cluster of 317 genes expressed independently of hypha formation, whereas 86 genes were dependent on mycelial development. Both sets revealed the activation of the sulfur-amino acid biosynthesis pathway as a feature of C. albicans biofilms.