Shrimp AHPND-causing plasmids encoding the PirAB toxins as mediated by pirAB-Tn903 are prevalent in various Vibrio species.
Shrimp AHPND-causing plasmids encoding the PirAB toxins as mediated by pirAB-Tn903 are prevalent in various Vibrio species.
复制标题
引起虾 AHPND 的质粒编码由 pirAB-Tn903 介导的 PirAB 毒素，在各种弧菌物种中普遍存在
DOI：
10.1038/srep42177
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发表时间：
2017-02-07
影响因子：
4.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Xiao J;Liu L;Ke Y;Li X;Liu Y;Pan Y;Yan S;Wang Y
急性肝胰腺坏死病（AHPND）是一种新出现的虾病，由副溶血性弧菌中发现的一个质粒所编码的pirAB毒素引起。pirAB毒素是与发光杆菌昆虫相关（Pir）毒素的同源物。在此，我们报道了从欧文氏弧菌中分离出的致AHPND质粒的完整序列，以及它的11个同源质粒（pVH家族）的序列。此外，我们还纳入了13个相关质粒（pVH - r家族），这些质粒不含从哈维氏弧菌分支内多种菌种中分离出的pirAB基因。再者，在pVH中鉴定出了pirAB - Tn903复合转座子，并且该转座子的两端似乎同时插入到祖先质粒的不同位点。在坎贝氏弧菌的一个非pVH质粒中也检测到了pirAB的同源对应物。综上所述，我们的研究结果为哈维氏弧菌分支中pirAB以及相关质粒的获取和进化提供了新的见解。
Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease caused by pirAB toxins encoded by a plasmid found in Vibrio parahaemolyticus. The pirAB toxins are the homologs of the Photorhabdus insect-related (Pir) toxins. Here, we report the complete sequences of the AHPND-causing plasmid isolated from V. owensii, as well as those of its 11 siblings (pVH family). In addition, we also included 13 related plasmids (pVH-r family) without the pirAB genes isolated from a variety of species within the Vibrio Harveyi clade. Furthermore, the pirAB-Tn903 composite transposon was identified in pVH, and both ends of the transposon appeared to have inserted simultaneously into the ancestor plasmid at different sites. The homologue counterparts of pirAB were also detected in a non-pVH plasmid in V. campbellii. Taken together, our results provide novel insights into the acquisition and evolution of pirAB as well as related plasmids in the Vibrio Harveyi clade.