ACO1, a gene for aminocyclopropane-1-carboxylate oxidase: effects on internode elongation at the heading stage in rice
ACO1, a gene for aminocyclopropane-1-carboxylate oxidase: effects on internode elongation at the heading stage in rice
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DOI：
10.1111/j.1365-3040.2009.02106.x
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发表时间：
2010-05-01
影响因子：
7.3
通讯作者：
Takano, M.
中科院分区：
文献类型：
--
作者：
Iwamoto, M.;Baba-Kasai, A.;Takano, M.
虽然报道了水稻（Oryza sativa L.）表明其高水平转录与淹水期间深水水稻的节间伸长相关，但ACO 1在水稻发育中的作用在很大程度上是未知的。ACO 1表达的组织特异性表明，其转录本在抽穗期伸长节间的下部显著积累。组织化学分析和原位杂交表明，ACO 1的表达定位于基部的叶鞘直接高于节点或下部的伸长节间。为了进一步研究ACO 1的作用，表征了ACO 1缺陷型（aco 1）和过表达型（ACO 1-OX）突变体。aco 1突变体的伸长节间的总长度略短于野生型植株，而ACO 1-OX突变体的伸长节间的总长度较长。有趣的是，ACC合酶基因ACS 1和乙烯信号基因OsEIN 2的表达上调aco 1突变体。本研究表明，ACO 1对抽穗期节间伸长的抑制作用不大，而ACS 1和OsEIN 2的表达上调可能减弱对节间伸长的抑制作用。
Although reports on a gene for 1-amino-cyclopropane-1-carboxylate (ACC) oxidase (ACO1) in rice (Oryza sativa L.) suggest that high levels of its transcript are associated with internode elongation of deep-water rice during submergence, the role of ACO1 in rice development is largely unknown. The tissue-specificity of ACO1 expression indicated that its transcript significantly accumulated in lower parts of elongating internodes at the heading stage. Histochemical analysis and in situ hybridization showed that the ACO1 expression was localized in the basal parts of leaf sheaths immediately above nodes or the lower parts of elongating internodes. To further examine the role of ACO1, ACO1-deficient (aco1) and overexpressing (ACO1-OX) mutants were characterized. The total length of the elongated internodes of aco1 mutants was slightly shorter than that of wild-type plants and that of ACO1-OX mutants was longer. Interestingly, expression of the ACC synthase gene ACS1 and ethylene signalling gene OsEIN2 was up-regulated in the aco1 mutants. This study suggests that the ACO1 has a little effect on internode elongation at the heading stage, and that up-regulation of the ACS1 and OsEIN2 expression may attenuate inhibition of internode elongation.