Star volume of marrow space and trabeculae of the first lumbar vertebra: sampling efficiency and biological variation.
Star volume of marrow space and trabeculae of the first lumbar vertebra: sampling efficiency and biological variation.
复制标题
第一腰椎骨髓空间和小梁的星体积：采样效率和生物学变异。
DOI：
10.1016/8756-3282(89)90140-3
复制
发表时间：
1989
期刊：
影响因子：
4.1
通讯作者：
F. Melsen
中科院分区：
文献类型：
--
作者：
A. Vesterby;H. Gundersen;F. Melsen
随着年龄的增长，骨小梁数量的减少不能仅仅用骨小梁变薄来解释，还必须是由于结构性骨小梁的损失导致骨小梁网络的不连续性。由于骨的复杂结构和各向异性，很难通过传统的组织形态测量法来证明这种结构变化。然而，当使用垂直剖面和专门设计的各向异性测试系统时，可以从各向异性结构获得无偏的体视估计量。这与骨结构的新的、无偏差的体视学参数相结合——星形体积对于阐明骨结构的组织学变化具有重要意义。星形体积被定义为从特定点向各个方向清晰可见的物体所有部分的平均体积，并取物体内部所有点的平均值。它被定义为任何类型的物体，包括像骨髓空间这样的空腔和像小梁系统这样的网络。使用框架和带有点和线的网格进行测量。研究的材料是从两名女性和六名男性身上获得的第一块腰椎，年龄在26至75岁之间，没有恶性或代谢性骨疾病。取样程序按照垂直截面的要求。结果确实显示，随着年龄的增长，骨髓空间的星形体积显着增加了五倍；对于小梁的星形体积，没有发现这种年龄相关性。骨髓空间大小增加的唯一解释是通过去除或穿孔骨小梁。总之，星形体积是一种有前途的估计量，并且可能对于阐明结构骨变化的发病机制具有重要意义；它是唯一能够对骨髓空间特征尺寸进行直接且体视学无偏估计的参数。为了确认其作为骨小梁结构变化参数的优越性，必须将其应用于包括骨质减少患者在内的更大群体，并与骨物理能力的直接估计器进行比较。
The decrease in the amount of trabecular bone which is seen with age cannot solely be explained by thinning of trabeculae but must also be due to a loss of structural trabecular bone leading to a discontinuity in the trabecular network. Due to the complex architecture and the anisotropy of bone it is difficult to demonstrate this structural change by conventional histomorphometry. Unbiased stereological estimators can however be obtained from anisotropic structures when using vertical sections and a specially designed anisotropic test system. This combined with a new and unbiased stereological parameter for bone structurethe star volumecan be of major importance in clarifying histological changes of bone structure. The star volume is defined as the mean volume of all the parts of an object which can be seen unobscured in all directions from a particular point with the mean value taken over all points inside the object. It is defined for any type of objects including cavities like marrow space and networks like the trabecular system. Measurements are performed using a frame and a grid with points and lines. The material investigated was the first lumbar vertebra obtained from two females and six males with ages 26 to 75 years without malignant or metabolic bone diseases. The sampling procedure was as required for vertical sections. Results did show a highly significant, five-fold increase in the star volume of the marrow space with age; no such age correlation was found for the star volume of the trabeculae. The only explanation for such an increase in the size of the marrow space is by removing or perforating trabecular bone.In conclusion, the star volume is a promising estimator and may be of importance in elucidating the pathogenesis of architectural bone changes; it is the only parameter which gives a direct and stereologically unbiased estimate of a characteristic size of the marrow space. To confirm its superiority as a parameter for structural changes in trabecular bone, it has to be applied to larger groups including patients with osteopenia and to be compared with direct estimators of physical competence of bone.