Endoglycoceramidase treatment inhibits synchronous oscillations of intracellular Ca2+ in cultured cortical neurons.
Endoglycoceramidase treatment inhibits synchronous oscillations of intracellular Ca2+ in cultured cortical neurons.
复制标题
神经酰胺内切酶处理可抑制培养的皮质神经元细胞内 Ca2 的同步振荡。
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Y. Kuroda
中科院分区：
文献类型：
--
作者：
K. Muramoto;M. Kawahara;K. Kobayashi;M. Ito;T. Yamagata;Y. Kuroda
神经节苷脂是神经细胞膜的主要成分，在突触区尤其丰富。为了评价内源性神经节苷脂在突触形成中的作用，使用神经酰胺糖苷内切酶（EGCase）从细胞表面去除神经节苷脂中的寡糖。我们以前曾报道过，培养的大鼠大脑皮层神经元之间的突触形成可以估计由Fura-2钙成像监测的突触活动的同步振荡。连续应用神经酰胺糖苷内切酶（EGCase）与其激活蛋白一起剂量依赖性地降低同步振荡的频率，而神经元及其轴突没有任何形态学变化。结果表明，在培养的皮层细胞表面与EGCase的鞘糖脂释放的寡糖是重要的皮层神经元之间的突触形成。
Gangliosides are major components of nerve cell membranes and are especially rich in synaptic areas. In order to evaluate the role of endogenous gangliosides in synapse formation, endoglycoceramidase (EGCase) was used to remove oligosaccharides of gangliosides from the cell surface. We have reported previously that synapse formation between cultured rat cerebral cortical neurons can be estimated by the synchronous oscillation of synaptic activity monitored by fura-2 calcium imaging. Continuous application of endoglycoceramidase (EGCase) together with its activator protein dose-dependently decreased the frequency of synchronous oscillations without any morphological changes in neurons and their neurites. The result suggests that oligosaccharides liberated from glycosphingolipids on cultured cortical cell surface with EGCase are important for synapse formation between cortical neurons.