Updated mitochondrial phylogeny of Pteriomorph and Heterodont Bivalvia, including deep-sea chemosymbiotic Bathymodiolus mussels, vesicomyid clams and the thyasirid clam Conchocele cf. bisecta
Updated mitochondrial phylogeny of Pteriomorph and Heterodont Bivalvia, including deep-sea chemosymbiotic Bathymodiolus mussels, vesicomyid clams and the thyasirid clam Conchocele cf. bisecta
复制标题
DOI：
10.1016/j.margen.2016.09.003
复制
发表时间：
2017-02-01
期刊：
影响因子：
1.9
通讯作者：
Yoshida, Takao
中科院分区：
文献类型：
--
作者：
Ozawa, Genki;Shimamura, Shigeru;Yoshida, Takao
双壳类的线粒体基因组常被用于比较基因组学和系统发育关系的研究。迄今为止，已经完成了100多个双壳类完整的线粒体基因组测序。然而，很少有线粒体基因组的深海化学共生双壳类，属于亚类Pteriomorphina和Heterodonta。在本研究中，我们对8种深海化学共生双壳类的线粒体基因组进行了测序：3种Bathymodiolus贻贝（B.你好，B。板额龙和B. septemdierum）、4种泡绵蛤（Abyssogena mariana，A. phaseoliformis，Isorropodon fossajaponicum，Phreagena okutanii，所有这些都是以前分类在Calyptogena属），和一种thyasirid clam（Conchocele cf. bisecta）。除了少数例外，这些线粒体基因组包含后生动物的典型基因：13个蛋白质编码基因，2个rRNA基因和22个tRNA基因。每个基因组中含有高A + T含量的主要非编码区，其中含有串联重复序列和发夹，被假设为控制区。根据14个共有基因的拼接序列构建了翼鱼纲和异齿亚目的系统发育树。Bathymodiolus与共生的贻贝科贻贝形成了一个单系Glade，囊蛤科蛤与帘蛤科形成了一个单系，C cf.在异齿亚目中基部的二裂分枝。已知双壳类的线粒体基因组的基因顺序不同。为了研究基因顺序的变化是否表现出系统发育的信号，树拓扑结构的基础上的最小数量的基因重排重建的两个分支（超家庭Tellinoidea，其中包括Psammobiidae，Semelidae，Solecurtidae，和Tellinidae;和沼泽包括Myidae，Mactridae，Arcticidae，Vesicomyidae，和Veneridae）具有高度的统计支持，在基于序列的遗传学。由此产生的树的拓扑结构几乎相同的序列为基础的树。我们目前的研究结果表明，双壳类的进化可以通过线粒体基因组的分析精确地追溯，这样的分析可能有助于理解双壳类的进化和多样性。(C)© 2016 Elsevier B. V.版权所有。
The mitochondrial genomes of bivalves have often been used for comparative genomics and for resolving phylogenetic relationships. More than 100 bivalve complete mitochondrial genomes have been sequenced to date. However, few mitochondrial genomes have been reported for deep-sea chemosymbiotic bivalves, which belong to the subclasses Pteriomorphia and Heterodonta. In the present study, we sequenced the mitochondrial genomes of eight deep-sea chemosymbiotic bivalve species: three species of Bathymodiolus mussels (B. japonicus, B. platifrons, and B. septemdierum), four species of vesicomyid clams (Abyssogena mariana, A. phaseoliformis, Isorropodon fossajaponicum, and Phreagena okutanii, all of which were formerly classified in the genus Calyptogena), and one species of thyasirid clam (Conchocele cf. bisecta). With a few exceptions, these mitochondrial genomes contained genes that are typical of metazoans: 13 protein-coding genes, two rRNA genes, and 22 tRNA genes. The major non-coding region with a high A + T content of each genome, which contained tandem repeats and hairpins, was hypothesized to function as a control region. The phylogenetic trees of Pteriomorphia and Heterodonta were reconstructed based on the concatenated sequences of 14 shared genes. Bathymodiolus formed a monophyletic Glade with asymbiotic Mytilidae mussels, the vesicomyid clams formed a monophyly that was sister to the Veneridae, and C cf. bisecta branched basally in the Heterodonta. It is known that the gene orders of mitochondrial genomes vary among bivalves. To examine whether gene order variation exhibits phylogenetic signals, tree topologies based on the minimum number of gene rearrangements were reconstructed for two clades (superfamily Tellinoidea, which includes the Psammobiidae, Semelidae, Solecurtidae, and Tellinidae; and the Glade comprising the Myidae, Mactridae, Arcticidae, Vesicomyidae, and Veneridae) with high statistical support in sequence-based phylogenies. The resulting tree topologies were almost identical to those of the sequence-based trees. Our present findings suggest that the evolution of bivalves could be precisely traced back through the analysis of mitochondrial genomes, and that such an analysis could contribute to understanding bivalve evolution and diversity. (C) 2016 Elsevier B.V. All rights reserved.