Effect of Lactobacillus salivarius on Th1/Th2 cytokines and the number of spleen CD4⁺ CD25⁺ Foxp3⁺ Treg in asthma Balb/c mouse.
Effect of Lactobacillus salivarius on Th1/Th2 cytokines and the number of spleen CD4⁺ CD25⁺ Foxp3⁺ Treg in asthma Balb/c mouse.
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发表时间：
2015-07
影响因子：
1.4
通讯作者：
Xiang Yun;Y. Shang;Miao Li
中科院分区：
文献类型：
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作者：
Xiang Yun;Y. Shang;Miao Li
背景支气管哮喘是一种慢性气道炎症性疾病，T淋巴细胞参与其中。方法采用卵白蛋白诱导Balb/c小鼠急性哮喘模型，观察唾液乳杆菌对哮喘小鼠行为学的影响，探讨唾液乳杆菌对哮喘小鼠Th 1/Th 2细胞因子及脾脏CD 4（+）CD 25（+）Foxp 3（+）Treg数量的影响。实时荧光定量PCR检测加塔-3 mRNA和T-bet mRNA的表达。流式细胞术检测CD 4（+）CD 25（+）Foxp 3（+）Treg/CD 4（+）比值。结果致敏前口服唾液乳杆菌可在一定程度上缓解哮喘小鼠的临床症状、气道高反应性和气道炎症;唾液乳杆菌可能通过在转录水平上增加T-bet mRNA的表达，同时抑制加塔-3 mRNA的表达，改善哮喘小鼠Th 1/Th 2失衡。结论CD 4（+）CD 25（+）Foxp 3（+）Treg细胞可能参与了支气管哮喘的发病过程，并可能是唾液乳杆菌改善Th 1/Th 2失衡的上游调节机制。
BACKGROUND Bronchial asthma is a chronic airway inflammatory disease that involves T lymphocytes. METHODS In order to explore the effect of Lactobacillus salivarius on Th1/Th2 cytokines and the number of spleen CD4(+) CD25(+) Foxp3(+) Treg in asthma Balb/c mouse, we constructed acute asthma model with ovalbumin to observe the mouse behavior change in Balb/c mice. The expression of GATA-3 mRNA and T-bet mRNA was measured by real-time PCR. The proportion of CD4(+) CD25(+) Foxp3(+) Treg/CD4(+) was determined by flow cytometry. RESULTS The results demonstrated that oral gavage with Lactobacillus salivarius before sensitization could alleviate the clinical symptoms, airway hyper-reactivity and airway inflammation in asthma mouse to some extent; Lactobacillus salivarius may improve the imbalance of Th1/Th2 in asthma mouse through increasing the expression of T-bet mRNA at the transcriptional level and inhibiting the expression of GATA-3 mRNA simultaneously. CONCLUSION CD4(+) CD25(+) Foxp3(+) Treg cells may be involved in the pathogenesis of bronchial asthma, and may be the upstream regulatory mechanism of the improvement of Th1/Th2 imbalance by Lactobacillus salivarius.