Serratia oryzae sp nov., isolated from rice stems
Serratia oryzae sp nov., isolated from rice stems
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米沙雷菌 (Serratia oryzae sp nov.)，从稻茎中分离出来
DOI：
10.1099/ijsem.0.002049
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发表时间：
2017
影响因子：
2.8
通讯作者：
Zhang Xiao-Xia
中科院分区：
文献类型：
--
作者：
Zhang Cai-Wen;Zhang Jun;Zhao Juan-Juan;Zhao Xia;Zhao Dong-Fang;Yin Hua-Qun;Zhang Xiao-Xia
从水稻茎杆中分离到一株新的内生细菌J11- 6 T。它的分类地位进行了调查，使用多相的方法。新菌株为革兰氏染色阴性、厌氧、能动、杆状。基于16 S rRNA基因序列的系统发育分析结果表明J11- 6 T为Rahnella属的成员，而基于pD、gyrB、rpoBandinfB基因序列的多位点序列分析（MLSA）表明J11- 6 T与Rahnellabut属的模式菌株有明显区别，表明它属于Serratia属的分支。MLSA系统发育分析结果表明，与该菌亲缘关系最近的是泉生沙雷氏菌和水生沙雷氏菌。主要的细胞脂肪酸是C16：1ω 7 c（38.7%）和C16：0（25.0%）。 DNA G+C含量为53.2mol%。J11 - 6菌株与水生拉恩氏菌CIP 78.65T菌株的DNA-DNA同源性为17.4%，与S菌株的DNA-DNA同源性为29.2%。 fonticolaLMG 7882 T，表明该菌株为沙雷氏菌属的一个新种。基因型和表型分析表明，J11- 6 T（=ACCC 19934 T =KCTC 52529 T）代表沙雷氏菌属的一个新种，其学名为Serratia oryzaesp.十一是建议。
A novel endophytic bacterium, strain J11-6T, was isolated from rice stems. Its taxonomic position was investigated using a polyphasic approach. The novel strain was Gram-staining-negative, facultatively anaerobic, motile and rod-shaped. Although the results of phylogenetic analysis based on 16S rRNA gene sequences indicated that J11-6Trepresented a member of the genusRahnella, multilocus sequence analysis (MLSA) on the basis of concatenated partialatpD, gyrB, rpoBandinfBgene sequences showed a clear distinction of J11-6Tfrom the type strains of species of the genusRahnellabut indicated that it lay within the clade of the genusSerratia. The phylogenetically closest species wereSerratia fonticolaandSerratia aquatilison the basis of the results of the MLSA phylogenetic analysis. The predominant cellular fatty acids were C16 : 1ω7c(38.7 %) and C16 : 0(25.0 %). The DNA G+C content was 53.2 mol%. The DNA–DNA relatedness was 17.4 % between J11-6TandRahnella aquatilisCIP 78.65T, and 29.2 % between J11-6TandS. fonticolaLMG 7882Twhich indicates that this strain represents a novel species of the genusSerratia. Characterization by genotypic and phenotypic analysis indicated that J11-6T(=ACCC 19934T=KCTC 52529T) represents a novel species of the genusSerratia, for which the nameSerratia oryzaesp. nov. is proposed.