Sleep is increased in mice with obesity induced by high-fat food
Sleep is increased in mice with obesity induced by high-fat food
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DOI：
10.1016/j.physbeh.2005.10.010
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发表时间：
2006-02-28
影响因子：
2.9
通讯作者：
Fang, JD
中科院分区：
文献类型：
--
作者：
Jenkins, JB;Omon, T;Fang, JD
白天过度嗜睡与人类肥胖有关。然而，关于肥胖动物睡眠的实验研究很少，结果也不一致。为了验证肥胖与睡眠增加有关的假设，我们研究了由高脂肪食物诱导的肥胖对小鼠睡眠的影响。我们首先确定了成年C57 BL/6小鼠（6个月大）的基线睡眠。在接下来的6周内，实验小鼠（n = 12）被转换为高脂肪食物，其中脂肪提供59%的卡路里，对照小鼠（n = 11）连续喂食常规实验室食物，其中脂肪提供16%的卡路里。高脂组大鼠体重逐渐增加，而对照组大鼠体重基本不变。在高脂肪喂养2周、4周和6周后评估时，觉醒程度有所减少。与此同时，非快速眼动睡眠（NREMS）大量增加（约80-100分钟/天）。快速眼动睡眠（REMS）没有改变。NREMS和REMS发作次数增加，而清醒发作的持续时间减少，主要是在黑暗期。在对照组中没有观察到这些睡眠变化。在高脂组，体重的增加与觉醒量的变化呈负相关，而与NREMS量的变化呈正相关，但能量摄入量的增加与觉醒量的变化无关。这些结果表明，肥胖动物在活动期睡眠压力增加，难以保持清醒。(c)2005年爱思唯尔公司All rights reserved.
Excessive daytime sleepiness has been associated with obesity in humans. However, experimental studies on sleep in obese animals are scarce and the results are not consistent. To test the hypothesis that obesity is associated with increased sleep, we examined the effects of obesity, induced by high-fat food, on sleep in mice. We first determined baseline sleep in adult C57BL/6 mice (6 months of age). In the following 6 weeks, the experimental mice (n = 12) were switched to high-fat food, in which fat provided 59% of calories, and the control mice (n = 11) were continuously fed with regular lab chows, in which fat provided 16% of calories. The body weights increased steadily in the high-fat group, but maintained constant in the controls. Wakefulness was reduced when assessed after 2, 4, and 6 weeks of high-fat feeding. Concurrently, there were large increases (about 80-100 min/day) in non-rapid eye movement sleep (NREMS). Rapid eye movement sleep (REMS) was not altered. The numbers of NREMS and REMS episodes were increased, whereas the duration of waking episodes was reduced, mainly during the dark period. These alterations in sleep were not observed in the controls. In the high-fat group,. the increases of body weight, but not the amounts of energy intake, were negatively correlated with the change in the amounts of wakefulness and positively correlated with the change in the amounts of NREMS. These results indicate that the obese animals have increased sleep pressure and difficulties in maintaining wakefulness during the active phase. (c) 2005 Elsevier Inc. All rights reserved.