Effect of different metal ions for submerged culture of Shiraia bambusicola
Effect of different metal ions for submerged culture of Shiraia bambusicola
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发表时间：
2011
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通讯作者：
X. Ling
中科院分区：
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作者：
X. Ling
目的研究不同浓度Cu2+、Fe3+、Cr3+和Ca2+对竹木菌丝生物量和次氯酸钠产量的影响。方法在27℃、130 r/min条件下，用不同浓度的金属离子培养96 h。结果当Cr3+浓度为0.005%时，生物量最高，次香菇素产量最高；Fe3+含量分别为0.003%和0.005%时有利于生物量和次氯酸钠产量；Ca2+浓度为0.03%时最适宜菌丝生长，0.005%时产量最高；当Cu2+添加量为0.03%时，生物量和次香菇素产量均达到最佳。结论在添加金属离子的条件下，菌丝体干重最高可达15.28 g/L，产率最高可达8.12 mg/g。
Objective To study the effect of different levels of Cu2+,Fe3+,Cr3+,and Ca2+ on mycelia biomass and the hypocrellin yield by submerged culture of Shiraia bambusicola.Methods The fungis were cultured under 27 ℃ and 130 r/min for 96 h with each level of metal ions.Results The biomass and the hypocrellin yield were the highest with Cr3+ of 0.005%;Fe3+ of 0.003% and 0.005% contents were favorable to the biomass and the hypocrellin yield,respectively;Ca2+ content of 0.03% was suitable for the mycelia growth and the hypocrellin yield benefited from the concentration of 0.005%;Cu2+ content of 0.03% was added,both the biomass and the hypocrellin yield reached the best.Conclusion The highest mycelia dry weight and the hypocrellin yield could reach 15.28 g/L and 8.12 mg/g,respectively in addition of metal ion.