PREPARATION OF C-11 LABELED ACETATE AND PALMITIC ACID FOR THE STUDY OF MYOCARDIAL-METABOLISM BY EMISSION-COMPUTERIZED AXIAL-TOMOGRAPHY
PREPARATION OF C-11 LABELED ACETATE AND PALMITIC ACID FOR THE STUDY OF MYOCARDIAL-METABOLISM BY EMISSION-COMPUTERIZED AXIAL-TOMOGRAPHY
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DOI：
10.1007/bf02518360
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
SELWYN, AP
中科院分区：
文献类型：
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作者：
PIKE, VW;EAKINS, MN;SELWYN, AP
已开发的方法用于标记乙酸和棕榈酸与正电子发射放射性核素，11 C（T=20.4分钟）。标记是通过将适当的烷基溴化镁（甲基溴化镁或正十五烷基溴化镁）与14 N（p，α）11 C核反应产生的11 C标记的二氧化碳碳酸化来实现的。每种标记方法的放射化学产率和速度使得获得放射化学纯的产品，其呈注射形式并且具有适于通过发射计算机化轴向断层摄影研究心肌代谢的活性（>10 mCi）。使用高压液相色谱法和薄层色谱法评估每种放射性药物的放射化学纯度。用酶法测定~（11）C标记乙酸盐的比活度大于0.5 Ci/μmole。
Methods have been developed for the labelling of acetate and palmitic acid with the positron-emitting radionuclide,11C (T=20.4 min). Labelling was achieved via carbonation of the appropriate alkyl magnesium bromide (methyl magnesium bromide or n-pentadecyl magnesium bromide) with11C-labelled carbon dioxide produced by the14N(p, α)11C nuclear reaction. The radiochemical yield and speed of each method of labelling are such that a radiochemically pure product is obtained in injectable form and in activity (>10 mCi) suitable for the study of myocardial metabolism by emission-computerised axial tomography. High pressure liquid chromatography and thin layer chromatography were used to assess the radiochemical purity of each radiopharmaceutical. The specific activity of11C-labelled acetate was estimated by an enzymic procedure to be greater than 0.5 Ci/μmole.