Rat limbal epithelial side population cells exhibit a distinct expression of stem cell markers that are lacking in side population cells from the central cornea
Rat limbal epithelial side population cells exhibit a distinct expression of stem cell markers that are lacking in side population cells from the central cornea
复制标题
DOI：
10.1016/j.febslet.2005.10.047
复制
发表时间：
2005-12
期刊：
影响因子：
3.5
通讯作者：
Terumasa Umemoto;M. Yamato;K. Nishida;C. Kohno;Joseph Yang;Y. Tano;T. Okano
中科院分区：
文献类型：
--
作者：
Terumasa Umemoto;M. Yamato;K. Nishida;C. Kohno;Joseph Yang;Y. Tano;T. Okano
侧群（SP）表型由各种组织和物种中的干细胞共享。在这里，我们证明了SP细胞与Hoechst染料流出，令人惊讶地收集从上皮细胞的大鼠利姆布斯和中央角膜，不像在人类和兔子的眼睛。我们的研究结果表明，大鼠角膜缘SP细胞有一个显着更高的表达干细胞标志物ABCG 2，巢蛋白，和缺口1，中央角膜SP细胞相比。免疫组化还显示，ABCG 2和上皮干/祖细胞标记物p63仅在基底角膜缘上皮细胞中表达。这些结果表明，ABCG 2表达与SP细胞的干细胞表型密切相关。
The side population (SP) phenotype is shared by stem cells in various tissues and species. Here we demonstrate SP cells with Hoechst dye efflux were surprisingly collected from the epithelia of both the rat limbus and central cornea, unlike in human and rabbit eyes. Our results show that rat limbal SP cells have a significantly higher expression of the stem cell markers ABCG2, nestin, and notch 1, compared to central corneal SP cells. Immunohistochemistry also revealed that ABCG2 and the epithelial stem/progenitor cell marker p63 were expressed only in basal limbal epithelial cells. These results demonstrate that ABCG2 expression is closely linked to the stem cell phenotype of SP cells.