A polymerase chain reaction-based linkage map of broccoli and identification of quantitative trait loci associated with harvest date and head weight
A polymerase chain reaction-based linkage map of broccoli and identification of quantitative trait loci associated with harvest date and head weight
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DOI：
10.21273/jashs.132.4.507
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发表时间：
2007-07-01
影响因子：
1.9
通讯作者：
Juvik, John A.
中科院分区：
文献类型：
--
作者：
Brown, Allan F.;Jeffery, Elizabeth H.;Juvik, John A.
以两种形态学差异较大的青花菜为材料，对216个基于聚合酶链反应的分子标记进行多态性筛选。SSP. italica Plenck）系、'VI-158'和“Brocolette Neri E.切斯普里奥59这些简单重复序列（SSR）和序列相关扩增多态性（SRAP）引物对产生69多态性，用于构建一个连锁图谱的青花菜从人口162 F，：3个家庭来自这两个线之间的交叉。产生了10个连锁群，跨越的距离为468 cM，平均间隔宽度为9.4 cM。该图被用来确定数量性状基因座（QTL）与收获日期，成熟度和头重在同一地点生长超过2年的人口的差异。成熟天数和头重的遗传力估计值分别为0.84和0.64。在第一年鉴定出4个与收获成熟度相关的QTL，其描述了55.6%的表型变异，其中2个QTL在第二年也被检测到，其描述了29.2%的表型变异。1999年共检测到5个与单穗重相关的QTL，占表型变异的71.8%。其中两个QTL占24%的表型变异的头重在2000年。据我们所知，这是第一张青花菜的连锁图谱，也是第一张B的SSR和SRAP组合图谱。这将为ssp特异性性状的遗传分析提供有用的工具。意大利语。
A set of 216 polymerase chain reaction-based molecular markers was screened for polymorphisms using two morphologically dissimilar broccoli (Brassica oleracea L. ssp. italica Plenck) lines, 'VI-158' and "Brocolette Neri E. Cespuglio'. Fifty-nine of these simple sequence repeat (SSR) and sequence-related amplified polymorphic (SRAP) primer pairs generated 69 polymorphisms that were used to construct a linkage map of broccoli from a population of 162 F,:3 families derived from the cross between these two lines. Ten linkage groups were generated that spanned a distance of 468 cM with an average interval width of 9.4 cM. The map was used to identify quantitative trait loci (QTL) associated with differences in harvest date maturity and head weight in the population grown in the same location over 2 years. Heritability estimates for days to maturity and head weight were 0.84 and 0.64, respectively. Four QTL for harvest maturity were identified that described 55.6% of the phenotypic variation in the first year with two of these QTL also detected in the second year of the experiment that described 29.2% of the phenotypic variation. Five QTL were identified as associated with head weight in 1999 and accounted for 71.8% of the phenotypic variability. Two of these QTL accounted for 24% of the phenotypic variability in head weight in 2000. To our knowledge, this is the first linkage map of broccoli and the first combined SSR and SRAP map of B. oleracea, which should provide a useful tool for the genetic analysis of traits specific to ssp. italica.