Enrichment of benzo[a]pyrene in vegetable oils and determination by HPLC-FL.
Enrichment of benzo[a]pyrene in vegetable oils and determination by HPLC-FL.
复制标题
植物油中苯并[a]芘的富集及HPLC-FL测定。
DOI：
10.1016/s0039-9140(00)00300-3
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发表时间：
2000
期刊：
影响因子：
6.1
通讯作者：
J. Lozano
中科院分区：
文献类型：
--
作者：
S. Troche;M. Falcón;S. G. Amigo;M. A. Yusty;J. Lozano
我们开发了一种测定植物油中致癌物质苯并[a]芘 (BP) 的简单方法。该方法包括在乙腈中萃取植物油，在旋转蒸发器中浓缩至干，以及将残余物重新溶解在己烷中。在 Sep-Pack Silica Plus 柱上纯化己烷提取物，并通过 HPLC-FL 测定分离提取物中的 BP。橄榄油的检测限和定量限分别为 0.23 和 0.32 μg kg−1。回收率 (>93%) 和 RSD (<4%) 令人满意。当应用于 18 个油样时，BP 水平从未检测到到 1.99 μg kg−1。
We have developed a simple method for the determination of the carcinogen Benzo[a]pyrene (BP) in vegetable oils. The method consists of extraction of the vegetable oil in acetonitrile, concentration to dryness in rotary evaporator and redissolution of the residue in hexane. The purification of the hexane extract was on Sep-Pack Silica Plus cartridges, and the determination of the BP in the isolated extract was by HPLC-FL. Detection and quantification limits were 0.23 and 0.32 μg kg−1of olive oil, respectively. Recovery (>93%) and RSD (<4%) were satisfactory. When applied to 18 oil samples, BP levels varied from not detected to 1.99 μg kg−1.