Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes
Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes
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DOI：
10.1016/j.ejcb.2022.151246
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发表时间：
2022-06-03
影响因子：
6.6
通讯作者：
Kotov，Alexei A.
中科院分区：
文献类型：
--
作者：
Adashev，Vladimir E.;Bazylev，Sergei S.;Kotov，Alexei A.
动物精子发生过程中体细胞和生殖细胞之间的紧密相互作用是保守的。黑腹果蝇的睾丸是鉴定成熟配子产生过程的首选模型。然而，体细胞孢囊细胞的分化过程和体细胞-胚系相互作用目前还没有得到充分研究。在这里，我们专注于早期和成熟的体细胞囊肿细胞的转录组表达模式的比较，以找出发生在他们的发育变化。我们采用了基于FACS的方法从果蝇睾丸中分离早期和成熟的体细胞囊肿细胞，随后制备RNA-Seq文库，并分析分选细胞中的基因差异表达。我们发现早期囊肿细胞中参与细胞周期相关过程的基因表达增加，这对于早期囊肿细胞，囊肿干细胞的关键群体的增殖和自我更新是必要的。在早期囊肿细胞中上调的基因中也检测到了为适当的囊肿形成而形成的板状伪足样突起组织所需的基因。基因本体论和相互作用组分析的上调基因在成熟的囊肿细胞揭示了一个惊人的过度代表性的基因类别负责代谢和分解代谢的细胞过程，以及基因支持的能量状态的细胞提供的氧化磷酸化是在线粒体中进行。我们对差异表达基因的比较分析揭示了早期和成熟囊肿细胞的主要特点，并为它们的调节提供了新的见解，这对男性生育力很重要。
The tight interaction between somatic and germline cells is conserved in animal spermatogenesis. The testes ofDrosophila melanogasterare the model of choice to identify processes responsible for mature gamete production. However, processes of differentiation and soma-germline interactions occurring in somatic cyst cells are currently understudied. Here we focused on the comparison of transcriptome expression patterns of early and mature somatic cyst cells to find out the developmental changes taking place in them. We employed a FACS-based approach for the isolation of early and mature somatic cyst cells from fly testes, subsequent preparation of RNA-Seq libraries, and analysis of gene differential expression in the sorted cells. We found increased expression of genes involved in cell cycle-related processes in early cyst cells, which is necessary for the proliferation and self-renewal of a crucial population of early cyst cells, cyst stem cells. Genes proposedly required for lamellipodium-like projection organization for proper cyst formation were also detected among the upregulated ones in early cyst cells. Gene Ontology and interactome analyses of upregulated genes in mature cyst cells revealed a striking over-representation of gene categories responsible for metabolic and catabolic cellular processes, as well as genes supporting the energetic state of the cells provided by oxidative phosphorylation that is carried out in mitochondria. Our comparative analyses of differentially expressed genes revealed major peculiarities in early and mature cyst cells and provide novel insight into their regulation, which is important for male fertility.