Penetration Study of Formulated Nanosized Titanium Dioxide in Models of Damaged and Sun-Irradiated Skins
Penetration Study of Formulated Nanosized Titanium Dioxide in Models of Damaged and Sun-Irradiated Skins
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DOI：
10.1111/j.1751-1097.2012.01181.x
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发表时间：
2012-11-01
影响因子：
3.3
通讯作者：
Duplan, Helene
中科院分区：
文献类型：
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作者：
Miquel-Jeanjean, Corinne;Crepel, Frederic;Duplan, Helene
无机紫外线过滤剂，如二氧化钛（TiO 2），可安全用于健康皮肤，通常用于受损和受辐射的皮肤。本研究的目的是评价防晒剂中所含TiO 2纳米颗粒（初级尺寸>= 20 nm）在完整、受损、辐照和受损/辐照猪皮中的体外皮肤渗透性。皮肤渗透和本地化的二氧化钛防晒霜应用后24小时进行了研究，定量使用电感耦合等离子体质谱法，定性使用透射电子显微镜（TEM）。纳米颗粒的元素特性通过TEM偶联的能量色散X射线分析（TEM-EDX）评估。在完整和受损/辐照皮肤中，在表面和角质层（SC）分别发现102.35 +/- 4.20%和102.84 +/- 5.67%的钛沉积，而在有活力的表皮和真皮中仅发现0.19 +/-0.15%和0.39 +/-0.39%，并且在受体流体中未检测到钛。TEM-EDX分析证实了在SC表面由TiO 2形成的聚集体中存在钛，正如防晒制剂中已经表征的那样。因此，包含在防晒剂中的TiO 2纳米颗粒保留在SC的最上层，无论是在完整的皮肤中还是在受损的和/或暴露于模拟太阳辐射的皮肤中。
Inorganic ultraviolet filters such as titanium dioxide (TiO2), safe to use on healthy skin, are often applied on compromised and irradiated skin. The aim of this study was to evaluate in vitro the cutaneous penetration of TiO2 nanoparticles (>= 20 nm primary size), included in a sunscreen, in intact, damaged, irradiated, and damaged/irradiated pigskin. Cutaneous penetration and localization of TiO2 after a 24-h sunscreen application were investigated quantitatively using inductively coupled plasma-mass spectrometry, and qualitatively using transmission electron microscopy (TEM). Elemental identity of the nanoparticles was evaluated by TEM-coupled Energy Dispersive X-ray analysis (TEM-EDX). In intact and damaged/irradiated skins, 102.35 +/- 4.20% and 102.84 +/- 5.67% of the titanium deposited, respectively, were found at the surface and stratum corneum (SC), whereas only 0.19 +/- 0.15% and 0.39 +/- 0.39% were found in the viable epidermis and dermis and no titanium was detected in the receptor fluid. TEM-EDX analysis confirmed the presence of titanium in the aggregates formed by TiO2 at the SC surface, as already characterized in the sunscreen formulation. TiO2 nanoparticles included in a sunscreen thus remain in the uppermost layers of the SC, whether in intact skin or in compromised and/or skin exposed to simulated solar radiation.