HPLC determination of phenylpropanolamine in pharmaceutical OTC preparations.
HPLC determination of phenylpropanolamine in pharmaceutical OTC preparations.
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HPLC 测定非处方药制剂中苯丙醇胺的含量。
DOI：
10.1002/bmc.186
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
A. Kaddoumi
中科院分区：
文献类型：
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作者：
K. Nakashima;Saori Kanehara;A. Kaddoumi
建立了一种简便的HPLC法测定市售非处方制剂中苯丙醇胺（PPA）、苯乙双胍（PE）和扑尔敏（CPA）的含量。用水或甲醇稀释，然后过滤并直接进样至HPLC系统中，制备样品溶液。移动的相为含有庚烷磺酸钠（0.5 mM）作为离子对的甲醇-乙腈-乙酸（0.1 M）-三乙胺（20：20：60：0.6，v/v/v/v）的混合物。采用反相ODS柱，检测波长254 nm。该化合物在2.5-1000 μ M范围内具有良好的线性，检测限为0.13 - 0.48 μ M。当与PPA一起存在时，PE、咖啡因和CPA很好地分离。该方法已用于药物制剂（包括硬胶囊和软胶囊）中PPA的测定。
A convenient HPLC method to determine phenylpropanolamine (PPA) in addition to phenylephrine (PE) and chlorpheniramine (CPA) in commercially available over-the-counter (OTC) preparations has been developed. Sample solutions were prepared by dilution with water or methanol followed by filtration and direct injection into the HPLC system. The mobile phase was a mixture of methanol-acetonitrile-acetic acid (0.1 M)-triethylamine (20:20:60:0.6, v/v/v/v) containing sodium heptanesulfonate (0.5 mM) as an ion pair. The separation was achieved on a reversed-phase ODS column with detection wavelength set at 254 nm. The compounds showed good linearity in the range 2.5-1000 micro M with detection limits ranged from 0.13 to 0.48 micro M. PE, caffeine and CPA were well separated when present together with PPA. The method was applied to the determination of PPA in pharmaceutical preparations including hard and soft capsules.