L- and P-selectin and CD11/CD18 in intracapillary neutrophil sequestration in rabbit lungs
L- and P-selectin and CD11/CD18 in intracapillary neutrophil sequestration in rabbit lungs
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DOI：
10.1164/ajrccm.159.1.9709011
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发表时间：
1999-01-01
影响因子：
24.7
通讯作者：
Doerschuk, CM
中科院分区：
文献类型：
--
作者：
Kubo, H;Doyle, NA;Doerschuk, CM
补体片段的输注诱导肺毛细血管内中性粒细胞的快速隔离。本研究通过超微结构免疫组织化学检测了这种隔离发生的机制，以及补体片段对l -选择素和CD11/CD18表达的影响。使用抗p -选择素抗体、岩藻胶蛋白、l -选择素耗尽中性粒细胞和抗CD18抗体的研究表明，中性粒细胞隔离不需要选择素和CD18。然而，维持肺毛细血管内隔离的中性粒细胞需要l -选择素和CD11/CD18。补体片段组肺毛细血管中性粒细胞表达l -选择素比生理盐水组低72%，CD11/CD18比生理盐水组高98%。l -选择素的脱落优先发生在质膜的微绒毛突，而不是在平坦的中间区域。15分钟后，约28%的l -选择素仍留在毛细血管内中性粒细胞膜上，可能可以结合。体内l -选择素的脱落速度比体外慢。这些研究表明，补体片段诱导的中性粒细胞隔离至少需要两个连续的步骤，一个不需要识别的粘附分子，第二个需要l -选择素和CD11/CD18。
Infusion of complement fragments induces rapid sequestration of neutrophils within pulmonary capillaries. This study examined the mechanisms through which this sequestration occurs, as well as the effect of complement fragments on the expression of L-selectin and CD11/CD18 using ultrastructural immunohistochemistry. Studies using anti-P-selectin antibodies, fucoidin, L-selectin-depleted neutrophils, and anti-CD18 antibodies showed that selectins and CD18 were not required for neutrophil sequestration. However, maintaining the sequestered neutrophils within the pulmonary capillaries required both L-selectin and CD11/CD18. Neutrophils in the pulmonary capillaries of rabbits given complement fragments expressed 72% less L-selectin and 98% more CD11/CD18 than did those in rabbits given saline. Shedding of L-selectin occurred preferentially from the microvillar processes of the plasma membrane rather than from the flat intervening regions. About 28% of L-selectin still remained on intracapillary neutrophil membranes after 15 min and was likely available for binding. Shedding of L-selectin appeared slower in vivo than in vitro. These studies indicate that neutrophil sequestration induced by complement fragments requires at least two sequential steps, one that does not require recognized adhesion molecules followed by a second that requires L-selectin and CD11/CD18.