Target‐Effector Interaction in the Natural Killer Cell System
Target‐Effector Interaction in the Natural Killer Cell System
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自然杀伤细胞系统中的靶标效应器相互作用
DOI：
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发表时间：
1978
影响因子：
3.7
通讯作者：
M. Klein
中科院分区：
文献类型：
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作者：
J. Roder;M. Klein
这些结果表明，在正常未免疫小鼠的淋巴器官中可检测到两个靶结合细胞（TBC）亚群。一种细胞类型不粘附于尼龙羊毛柱，并选择性地结合大量易受自然杀伤（NK）细胞裂解的肿瘤细胞靶。随着年龄的增长，这些尼龙非粘附TBC的频率的上升和下降与这些小鼠中的NK细胞活性密切相关。尼龙非粘附TBC是特异性的，因为它们可以被敏感靶点的亚细胞超声抑制，但不敏感靶点则不能。这些TBC在裸鼠中的存在及其通过尼龙羊毛柱的能力与以下建议相一致，即与NK细胞一样，它们可能不是成熟的T细胞、巨噬细胞或B细胞，因此代表一种独特但尚未确定的淋巴细胞亚群。控制TBC频率的基因以显性方式遗传，并与H-2区域相关。群体中TBC的频率与溶解水平之间的强相关性提供了强有力的间接证据，表明TBC可能代表NK细胞或与NK细胞密切相关。相比之下，第二种细胞类型（尼龙粘附群体）不受任何可检测的遗传控制，并与靶标非特异性结合。此外，这些尼龙粘附TBC与尼龙非粘附TBC的不同之处在于，它们与溶解、年龄变化和器官分布缺乏相关性。我们相信这些观察结果为最终理解NK细胞系统中涉及的靶结构和受体提供了基础。
These results show that two subpopulations of target‐binding cells (TBC) can be detected in the lymphoid organs of normal, nonimmunized mice. One cell type is not adherent to nylon wool columns and binds selectively to a large number of tumour cell targets which are susceptible to lysis by the natural killer (NK) cell. The rise and fall in the frequency of these nylon nonadherent TBC, with age, closely parallels the NK cell activity in these mice. Nylon nonadherent TBC were specific since they could be inhibited by subcellular sonicates of sensitive targets but not insensitive targets. The presence of these TBC in nude mice and their ability to pass through nylon wool columns is compatible with the suggestion that, like the NK cell, they may not be mature T cells, macrophages or B cells and hence represent a distinct but not yet defined subpopulation of lymphocytes. The genes controlling the frequency of TBC are inherited in a dominant fashion and are linked to the H‐2 region. The strong correlation between the frequency of TBC in a population and the level of lysis provides strong indirect evidence that the TBC may represent, or be closely related to, the NK cell. In contrast, the second cell type(s), a nylon‐adherent population, was not subject to any detectable genetic control and bound to targets nonspecifically. Furthermore, these nylon‐adherent TBC differed from nylon‐nonadherent TBC in their lack of correlation with lysis, age variations and organ distribution. We believe these observations provide the basis for the eventual understanding of the target structures and receptors involved in the NK cell system.