GLUCOSE-METABOLISM IN THE EXTREME THERMOACIDOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS
GLUCOSE-METABOLISM IN THE EXTREME THERMOACIDOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS
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DOI：
10.1042/bj2240407
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发表时间：
1984-01-01
影响因子：
4.1
通讯作者：
BUONOCORE, V
中科院分区：
文献类型：
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作者：
DEROSA, M;GAMBACORTA, A;BUONOCORE, V
硫磺硫化叶菌（Sulfolobus solfataricus）是一种嗜热古细菌，能够在87 ℃和pH 3.5下以葡萄糖作为唯一碳源生长。生物体通过两种主要途径代谢葡萄糖。第一种途径涉及ATP依赖性磷酸化以产生葡萄糖6-磷酸，其容易异构化为果糖6-磷酸。在第二种途径中，葡萄糖通过NAD+依赖性脱氢转化为葡萄糖酸盐;葡萄糖酸盐然后脱水为2-酮基-3-脱氧葡萄糖酸盐，其继而裂解为丙酮酸盐和甘油醛。每个代谢步骤已在体外进行了测试，在70摄氏度的透析匀浆或部分纯化的馏分;已评估的最低要求的单一酶。中间体的鉴别基于色谱、光谱和/或合成证据以及特定的酶测定。葡萄糖氧化分解为丙酮酸在S。solfataricus与Entner-Doudoroff模式的不同之处在于不存在任何磷酸化步骤。
Sulfolobus solfataricus is a thermophilic archaebacterium able to grow at 87 degrees C and pH 3.5 on glucose as sole carbon source. The organism metabolizes glucose by two main routes. The first route involves an ATP-dependent phosphorylation to give glucose 6-phosphate, which readily isomerizes to fructose 6-phosphate. In the second route, glucose is converted into gluconate by an NAD+-dependent dehydrogenation; gluconate is then dehydrated to 2-keto-3-deoxygluconate, which, in turn, is cleaved to pyruvate and glyceraldehyde. Each metabolic step has been tested in vitro at 70 degrees C on dialysed homogenates or partially purified fractions; minimal requirements of single enzymes have been evaluated. Identification of the intermediates is based on chromatographic, spectroscopic and/or synthetic evidence and on specific enzymic assays. The oxidative breakdown of glucose to pyruvate occurring in S. solfataricus differs from the Entner-Doudoroff pattern in that there is an absence of any phosphorylation step.