A novel glucose biosensor platform based on Ag@AuNPs modified graphene oxide nanocomposite and SERS application
A novel glucose biosensor platform based on Ag@AuNPs modified graphene oxide nanocomposite and SERS application
复制标题
DOI：
10.1016/j.jcis.2013.06.007
复制
发表时间：
2013-09-15
影响因子：
9.9
通讯作者：
Ustundag, Zafer
中科院分区：
文献类型：
--
作者：
Gupta, Vinod Kumar;Atar, Necip;Ustundag, Zafer
本研究提出了一种新型的基于硫代苯基硼酸(MBA)封端的Ag@AuNPs/氧化石墨烯(Ag@AuNPs-GO)纳米材料的葡萄糖生物传感器的模板演示。用透射电子显微镜、X射线光电子能谱和X射线衍射仪对所制备的纳米复合材料进行了表征。透射电子显微镜照片显示，纳米复合材料中的Ag@AuNPs的直径在10-20 nm之间。将该纳米复合材料用于葡萄糖二醇基团与硼酸的络合测定。因此，将葡萄糖氧化酶(GOD)固定在MBA端基的Ag@AuNPs-GO纳米复合薄膜(MBA-Ag@AuNPs-GO)中，制备了一种新型的葡萄糖生物传感器。葡萄糖的线性范围为2~6 mm，检出限为0.33 mm。所研制的生物传感器还成功地应用于血样中葡萄糖的测定。重复6次测量，计算出血样葡萄糖浓度为1.97+/-0.002 mm。(C)2013 Elsevier Inc.保留所有权利。
This study represents a novel template demonstration of a glucose biosensor based on mercaptophenyl boronic acid (MBA) terminated Ag@AuNPs/graphene oxide (Ag@AuNPs-GO) nanomaterials. The nanocomposites were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) method. The TEM image shows that Ag@AuNPs in the nanocomposite is in the range of diameters of 10-20 nm. The nanocomposite was used for the determination of glucose through the complexation between boronic acid and diol groups of glucose. Thus, a novel glucose biosensor was further fabricated by immobilizing glucose oxidase (GOD) into MBA terminated Ag@AuNPs-GO nanocomposite film (MBA-Ag@AuNPs-GO). The linearity range of glucose was obtained as 2-6 mM with detection limit of 0.33 mM. The developed biosensor was also applied successfully for the determination of glucose in blood samples. The concentration value of glucose in blood samples was calculated to be 1.97 +/- 0.002 mM from measurements repeated for six times. (C) 2013 Elsevier Inc. All rights reserved.