Virus inactivation during coagulation with aluminum coagulants
Virus inactivation during coagulation with aluminum coagulants
复制标题
DOI：
10.1016/j.chemosphere.2011.06.083
复制
发表时间：
2011-10-01
期刊：
影响因子：
8.8
通讯作者：
Ohno, Koichi
中科院分区：
文献类型：
--
作者：
Matsushita, Taku;Shirasaki, Nobutaka;Ohno, Koichi
我们使用噬菌体Q β和MS2来确定在凝固过程中铝凝固剂是否灭活病毒。我们用含病毒的溶液进行了分批凝聚和过滤实验。通过孔径为50 nm的膜过滤凝固溶液的上清液后，我们通过空斑形成单位（PFU）和聚合酶链反应（PCR）方法测量病毒浓度。无论感染性如何，通过PFU方法（用于测定感染性病毒浓度）测定的病毒浓度始终低于通过基于PCR的方法（用于测定总病毒浓度）测定的病毒浓度。这种差异可以通过由几个病毒颗粒组成的聚集体的形成或通过凝固过程中病毒的灭活来解释。前一种可能性可以忽略，因为（i）几种病毒颗粒的聚集体不会通过滤膜的50 nm孔，（ii）我们的粒度测量显示膜滤液中的病毒颗粒是单分散的。这些观察结果清楚地表明，在用铝凝结剂凝结过程后，非感染性Q β颗粒存在于膜滤液中。我们随后发现，在凝固过程中，病毒与水解铝物质混合后失去了感染性。(C)2011爱思唯尔有限公司保留所有权利。
We used the bacteriophages Q beta and MS2 to determine whether viruses are inactivated by aluminum coagulants during the coagulation process. We performed batch coagulation and filtration experiments with virus-containing solutions. After filtering the supernatant of the coagulated solution through a membrane with a pore size of 50 nm, we measured the virus concentration by both the plaque forming unit (PFU) and polymerase chain reaction (PCR) methods. The virus concentration determined by the PFU method, which determines the infectious virus concentration, was always lower than that determined by the PCR-based method, which determines total virus concentration, regardless of infectivity. This discrepancy can be explained by the formation of aggregates consisting of several virus particles or by the inactivation of viruses in the coagulation process. The former possibility can be discounted because (i) aggregates of several virus particles would not pass through the 50-nm pores of the filtration membrane, and (ii) our particle size measurements revealed that the virus particles in the membrane filtrate were monodispersed. These observations clearly showed that non-infectious Q beta particles were present in the membrane filtrate after the coagulation process with aluminum coagulants. We subsequently revealed that the viruses lost their infectivity after being mixed with hydrolyzing aluminum species during the coagulation process. (C) 2011 Elsevier Ltd. All rights reserved.