Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family
Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family
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DOI：
10.1016/s0014-5793(98)01130-2
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发表时间：
1998-10-02
期刊：
影响因子：
3.5
通讯作者：
Prévost, G
中科院分区：
文献类型：
--
作者：
Gravet, A;Colin, DA;Prévost, G
从金黄色葡萄球菌纽曼株中鉴定出一个新成员Luke(32 KDa)和LukD(34.3 kDa)，Luke与S类蛋白的同源性为58-68%，而LukD与E类蛋白的同源性为71-77%。免疫沉淀法和基因探测证实，在人类临床分离株中有30%的频率，与其他已知的白细胞毒素的分布不同(P<；0.005)。Luke+LukD与潘通-瓦伦丁杀白素一样，对兔皮肤有诱导皮肤坏死的作用，但与金黄色葡萄球菌所表达的其他亮氨酸毒素相比，没有溶血作用，且毒性较差。(C)1998年欧洲生化学会联合会。
A new member of the staphylococcal bi-component leucotoxins family, LukE (32 kDa) and LukD (34.3 kDa) has been characterized from Staphylococcus aureus strain Newman, LukE was 58-68% identical with the class S proteins, whereas LukD was 71-77% identical with the class E proteins of the family. A partial immunoreactivity with the various affinity-purified antibodies specific for the other proteins was observed, Immunoprecipitation assay and gene probing confirmed a 30% frequency among human clinical isolates, differing from the distribution of the other known leucotoxins (P < 0.005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus. (C) 1998 Federation of European Biochemical Societies.