Nucleotide sequence 5' of the chicken c-myc coding region: localization of a noncoding exon that is absent from myc transcripts in most avian leukosis virus-induced lymphomas.
Nucleotide sequence 5' of the chicken c-myc coding region: localization of a noncoding exon that is absent from myc transcripts in most avian leukosis virus-induced lymphomas.
复制标题
鸡 c-myc 编码区的 5 核苷酸序列：大多数禽白血病病毒诱导的淋巴瘤中 myc 转录物中不存在的非编码外显子的定位。
DOI：
10.1073/pnas.81.15.4697
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Hayward,WS
中科院分区：
文献类型：
--
作者：
Shih,CK;Linial,M;Goodenow,MM;Hayward,WS
我们已经确定了鸡c-myc编码外显子上游的2.2-磷酸酶对区域的核苷酸序列。使用RNA印迹分析，我们已经定位了一个非编码外显子的一个区域，该区域由700-800个碱基对的内含子与c-myc编码序列分开。在大多数禽白血病病毒诱导的淋巴瘤中，前病毒整合发生在第一外显子内或下游，因此可能取代了通常控制c-myc表达的调控序列。超过70%的整合位点聚集在第一内含子中的250个碱基对区域中，紧接在编码序列之前。在这些淋巴瘤中，myc基因的转录本中没有上游非编码外显子的序列;正常c-myc等位基因的RNA转录本没有以可检测的水平表达。
We have determined the nucleotide sequence of the 2.2-kilobase-pair region upstream of the chicken c-myc coding exons. Using RNA blot analysis, we have localized a noncoding exon to a region that is separated from the c-myc coding sequences by an intron of 700-800 base pairs. In most avian leukosis virus-induced lymphomas proviral integration has occurred within, or downstream of, the first exon, thus presumably displacing the regulatory sequences that normally control c-myc expression. More than 70% of the integration sites were clustered in a 250-base-pair region in the first intron, immediately preceding the coding sequences. Sequences from the upstream noncoding exon were absent from the myc transcripts in these lymphomas; RNA transcripts from the normal c-myc allele were not expressed at detectable levels.