Analysis of the hibernation cycle using LC-MS-based metabolomics in ground squirrel liver
Analysis of the hibernation cycle using LC-MS-based metabolomics in ground squirrel liver
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DOI：
10.1152/physiolgenomics.90323.2008
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发表时间：
2009-03-01
影响因子：
4.6
通讯作者：
Carey, Hannah V.
中科院分区：
文献类型：
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作者：
Nelson, Clark J.;Otis, Jessica P.;Carey, Hannah V.
纳尔逊CJ，奥蒂斯JP，马丁SL，凯里HV。使用基于LC-MS的代谢组学分析地松鼠肝脏中的冬眠周期。Physiol Genomics 37：43-51，2009.首次发表于2008年12月23日; doi：10.1152/physiolgenomics.90323.2008。- 哺乳动物冬眠的一个标志是代谢的灵活性，其典型特征是可逆的代谢抑制（休眠）和从夏季到冬季的主要碳水化合物到脂质代谢的季节性转变。为了提供对冬眠的控制和后果的新见解，我们使用基于LC/MS的代谢组学来测量地松鼠肝脏中小分子在五种活动状态下的差异：夏季，进入休眠，晚期休眠，从休眠中唤醒，以及间歇唤醒。在酶辅因子代谢、氨基酸代谢酶、嘌呤和嘧啶代谢中，季节性和觉醒周期内都有显著变化，观察到的代谢物在觉醒时增加，在觉醒时减少。多种脂质也发生了变化，包括1-oleoyllyso-磷脂酰胆碱，胆固醇硫酸盐，鞘氨醇，这往往是最低的在麻木，十六烷二酸，这积累在一个麻木回合。结果揭示了几类代谢物中发生的巨大变化，凸显了代谢组学分析在解读冬眠表型方面的价值。
Nelson CJ, Otis JP, Martin SL, Carey HV. Analysis of the hibernation cycle using LC-MS-based metabolomics in ground squirrel liver. Physiol Genomics 37: 43-51, 2009. First published December 23, 2008; doi: 10.1152/physiolgenomics.90323.2008. - A hallmark of hibernation in mammals is metabolic flexibility, which is typified by reversible bouts of metabolic depression ( torpor) and the seasonal shift from predominantly carbohydrate to lipid metabolism from summer to winter. To provide new insight into the control and consequences of hibernation, we used LC/MS-based metabolomics to measure differences in small molecules in ground squirrel liver in five activity states: summer, entering torpor, late torpor, arousing from torpor, and interbout arousal. There were significant alterations both seasonally and within torpor-arousal cycles in enzyme cofactor metabolism, amino acid catabolism, and purine and pyrimidine metabolism, with observed metabolites reduced during torpor and increased upon arousal. Multiple lipids also changed, including 1-oleoyllyso-phosphatidylcholine, cholesterol sulfate, and sphingosine, which tended to be lowest during torpor, and hexadecanedioic acid, which accumulated during a torpor bout. The results reveal the dramatic alterations that occur in several classes of metabolites, highlighting the value of metabolomic analyses in deciphering the hibernation phenotype.