IMMUNOCYTOCHEMICAL LOCALIZATION OF ESTRADIOL AND PROGESTERONE RECEPTORS IN THE MONKEY OVARY THROUGHOUT THE MENSTRUAL-CYCLE
IMMUNOCYTOCHEMICAL LOCALIZATION OF ESTRADIOL AND PROGESTERONE RECEPTORS IN THE MONKEY OVARY THROUGHOUT THE MENSTRUAL-CYCLE
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DOI：
10.1210/endo-123-6-2896
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发表时间：
1988-12-01
期刊：
影响因子：
4.8
通讯作者：
BRENNER, RM
中科院分区：
文献类型：
--
作者：
HILDPETITO, S;STOUFFER, RL;BRENNER, RM
在不同物种中，雌二醇和孕酮都可能在局部发挥作用，以调节卵巢功能。这项研究考察了雌激素和孕酮受体(分别为ER和PR)在月经周期中在灵长类卵巢的分布。卵巢取自早、中、晚期(n=3-6个/期)恒河猴或食蟹猴的卵泡和黄体期。用抗ER(H_222和D_(75))和PR(J_ZB_(39))的特异性单抗进行间接免疫细胞化学定位。通过比较与受体抗体或非特异性抗体孵育的相邻组织切片确定的特异性免疫细胞化学染色完全是核的。雌激素受体和孕激素受体在卵巢生发上皮中均有表达。无论发育到什么阶段，卵巢的其他结构(如间质、卵泡、间质组织或黄冠区)均未检测到ER。然而，在其他雌激素反应组织中也检测到ER，如猴的输卵管和假孕兔的黄铜。在猴卵巢中，正常卵泡和闭锁卵泡的间质和间质组织以及内膜和外膜在周期的各个阶段均可检测到PR。部分原始卵泡和初级卵泡的颗粒细胞表达PR。然而，发育超过初级阶段的卵泡颗粒层始终为PR阴性。只有在黄体生成素高峰后出现黄体化迹象的排卵前大卵泡的颗粒层显示PR的染色与卵泡膜中的相同。猕猴黄体呈PR特异性核染色。此外，黄体受体阳性核的百分比在早期(28.+-)之间变化(P<；0.05)。3%)，中(48.%+-)。1%)-和延迟(4.+-。2%)黄体期。无功能(血清孕酮0.5 ng/ml)退行性黄斑病变未见PR染色。PR阳性的黄体细胞也含有组织化学可检测到的3-β-羟基类固醇脱氢酶。这些数据与受体介导的孕激素的自分泌或旁分泌作用的概念是一致的，而不是雌激素在月经周期中灵长类卵巢的配子发生和内分泌功能中的作用。
Both estradiol and progesterone may act locally to modulate ovarian function in various species. This study examined the distribution of estradiol and progesterone receptors (ER and PR, respectively) within the primate ovary throughout the menstrual cycle. Ovaries were collected from rhesus or cynomolgus monkeys during the early, mid-, and late (n = 3-6/stage) follicular and luteal phases of the cycle. The tissues were processed for indirect immunocytochemical localization of receptors with specific monoclonal antibodies against ER (H222 and D75) and PR (JZB39). Specific immunocytochemical staining, as determined by comparing adjacent tissue sections incubated with either receptor antibodies or a nonspecific antibody, was exclusively nuclear. Both ER and PR were localized in the germinal epithelium of ovaries at all stages of the cycle. ER was not detected in any other ovarian structure (i.e. stroma, follicles, interstitial tissue, or copora lutea) regardless of the stage of development. However, ER was detected in other estrogen-responsive tissues, e.g. the oviduct of the monkey and copora lutea of the pseudopregnant rabbit. In the monkey ovary, PR was detected in stromal and interstitial tissues as well as theca interna and externa of healthy and atretic follicles at all stages of the cycle. The granulosa cells of some primordial and primary follicles demonstrated staining for PR. However, the granulosa layer of follicles that developed beyond the primary stage were consistently negative for PR. Only the granulosa layer of large preovulatory follicles that showed signs of luteinization after the LH surge showed staining for PR equivalent to that in the theca. Monkey corpora lutea exhibited specific nuclear staining for PR. Moreover, the percentage of receptorpositive nuclei in the corpus luteum varied (P < 0.05) between the early (28 .+-. 3%), mid (48 .+-. 1%)-, and late (4 .+-. 2%) luteal phase of the cycle. Nonfunctional (serum progesterone < 0.5 ng/ml) regressing copora lutea did not exhibit for staining for PR. Luteal cells that were PR positive also contained histochemically detectable 3.beta.-hydroxysteroid dehydrogenase. These data are consistent with the concept of a receptor-mediated autocrine or paracrine role for progestins, but not estrogens in the gametogenic and endocrine functions of the primate ovary throughout the menstrual cycle.