UNUSUAL IMMUNOGLOBULIN GENE REARRANGEMENT LEADS TO REPLACEMENT OF RECOMBINATIONAL SIGNAL SEQUENCES
UNUSUAL IMMUNOGLOBULIN GENE REARRANGEMENT LEADS TO REPLACEMENT OF RECOMBINATIONAL SIGNAL SEQUENCES
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DOI：
10.1126/science.3140378
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发表时间：
1988-10-14
期刊：
影响因子：
56.9
通讯作者：
DESIDERIO, SV
中科院分区：
文献类型：
--
作者：
MORZYCKAWROBLEWSKA, E;LEE, FEH;DESIDERIO, SV
观察到意想不到的免疫球蛋白基因重排，即信号序列替换，其中VH基因片段的重组信号序列完整地融合到DJH元件的5''末端。核苷酸不会从信号序列中丢失，但可能会从 DJH 编码序列中丢失。信号序列替换可能是由 VH 到 DJH 重组中中间体的替代解析引起的。这种类型的重排提供了一种改变免疫球蛋白基因片段靶向的方法，并提出了体内 VH-JH 连接发生的机制。信号序列替换可能代表产生抗体多样性的额外途径。
An unexpected immunoglobulin gene rearrangement, signal sequence replacement, was observed in which the recombinational signal sequences of a VH gene segment are fused intact to the 5'' end of a DJH element. Nucleotides are not lost from the signal sequences, but they may be lost from the DJH coding sequence. Signal sequence replacement may result from the alternative resolution of an intermediate in VH-to-DJH recombination. This type of rearrangement provides a means to alter the targeting of immunoglobulin gene segments and suggests a mechanism for the occurrence of VH-JH junctions in vivo. Signal sequence replacement may represent an additional pathway for the generation of antibody diversity.