Graft-versus-leukemia effects associated with detectable Wilms tumor-1-specific T lymphocytes after allogeneic stem-cell transplantation for acute lymphoblastic leukemia
Graft-versus-leukemia effects associated with detectable Wilms tumor-1-specific T lymphocytes after allogeneic stem-cell transplantation for acute lymphoblastic leukemia
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DOI：
10.1182/blood-2007-03-076844
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发表时间：
2007-09-15
期刊：
影响因子：
20.3
通讯作者：
Barrett, A. John
中科院分区：
文献类型：
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作者：
Rezvani, Katayoun;Yong, Agnes S. M.;Barrett, A. John
为了确定白血病相关肾母细胞瘤抗原 (WT') 是否会导致急性淋巴细胞白血病 (ALL) 异基因干细胞移植 (SCT) 后的移植物抗白血病 (GVL) 效应，我们研究了 10 名人类淋巴细胞抗原 (HLA)-A*0201 阳性 ALL 患者在 SCT 后免疫恢复早期阶段 (天) 对 WT1 的 CD8(+) T 细胞反应。 30-120）。在 SCT 之前，通过定量逆转录聚合酶链反应，10 名患者中有 7 名的外周血 (PB) 中可检测到 WT1 表达。使用 WT1/HLA-A*0201 四聚体和细胞内干扰素-γ (IFN-γ) 染色，仅在 SCT 前可检测到 WT1 表达的患者中发现 SCT 后的 WT1(+) CD8+ T 细胞应答（7 例中的 5 例 vs 31 例中的 0 例；P
To determine whether the leukemia-associated Wilms tumor antigen (WT') contributes to a graft-versus-leukemia (GVL) effect after allogeneic stem-cell transplantation (SCT) for acute lymphoblastic leukemia (ALL), we studied CD8(+) T-cell responses to WT1 in 10 human lymphocyte antigen (HLA)-A*0201-positive ALL patients during the early phase of immune recovery after SCT (days 30-120). Seven of 10 patients had detectable WT1 expression in their peripheral blood (PB) before SCT by quantitative reverse-transcription polymerase chain reaction. Using WT1/HLA-A*0201 tetramers and intracellular interferon-gamma (IFN-gamma) staining, WT1(+) CD8+ T-cell responses after SCT were found only in patients with detectable WT1 expression before SCT (5 of 7 vs. 0 of 31; P