Calcium Imaging of Basal Forebrain Activity during Innate and Learned Behaviors.
Calcium Imaging of Basal Forebrain Activity during Innate and Learned Behaviors.
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DOI：
10.3389/fncir.2016.00036
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发表时间：
2016
影响因子：
3.5
通讯作者：
Dan Y
中科院分区：
文献类型：
--
作者：
Harrison TC;Pinto L;Brock JR;Dan Y
基底前脑（BF）在唤醒、注意和记忆中起着至关重要的作用，其损伤与各种认知缺陷有关。BF由胆碱能、GABA能和谷氨酸能神经元组成。BF胆碱能神经元的电或光遗传刺激增强了皮层处理和行为表现，但这些细胞在行为过程中的自然活动才刚刚开始被表征。对GABA能和谷氨酸能神经元的了解更少。在这里，我们进行了BF神经元的显微内窥镜钙成像的小鼠从事自发行为，在他们的家笼（先天）或进行了去/不去听觉辨别任务（学习）。胆碱能神经元在运动过程中持续兴奋，包括跑步和舔，但GABA能和谷氨酸能神经元表现出不同的反应。所有类型的细胞都被公开的惩罚激活，无论是在识别任务的内部还是外部。这些发现揭示了BF细胞类型在自发行为和任务相关行为中的功能相似性和区别。
The basal forebrain (BF) plays crucial roles in arousal, attention, and memory, and its impairment is associated with a variety of cognitive deficits. The BF consists of cholinergic, GABAergic, and glutamatergic neurons. Electrical or optogenetic stimulation of BF cholinergic neurons enhances cortical processing and behavioral performance, but the natural activity of these cells during behavior is only beginning to be characterized. Even less is known about GABAergic and glutamatergic neurons. Here, we performed microendoscopic calcium imaging of BF neurons as mice engaged in spontaneous behaviors in their home cages (innate) or performed a go/no-go auditory discrimination task (learned). Cholinergic neurons were consistently excited during movement, including running and licking, but GABAergic and glutamatergic neurons exhibited diverse responses. All cell types were activated by overt punishment, either inside or outside of the discrimination task. These findings reveal functional similarities and distinctions between BF cell types during both spontaneous and task-related behaviors.