Unequal chromosome division and inter-genomic translocation occurred in somatic cells of wheat–rye allopolyploid
Unequal chromosome division and inter-genomic translocation occurred in somatic cells of wheat–rye allopolyploid
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DOI：
10.1007/s10265-011-0432-z
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发表时间：
2011-06
影响因子：
2.8
通讯作者：
Zong-xiang Tang;S. Fu;B. Yan;Huaiqiong Zhang;Z. Ren
中科院分区：
文献类型：
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作者：
Zong-xiang Tang;S. Fu;B. Yan;Huaiqiong Zhang;Z. Ren
通过基因组原位杂交研究了新合成的小麦-黑麦异源多倍体的第一、第二、第三和第四代异源多倍体。在CA4.4.7（S2代）的12个根尖细胞中观察到染色体间和染色体内连接，并且在5个根尖细胞中还检测到小麦和黑麦染色体之间的易位。在CA4.4.7.5和CA4.4.7.2.2(S3和S4代)的根尖细胞中，染色体连接再次发生，在CA4.4.7.5的7个细胞中检测到解离的小黑麦片段。在植物MSV6.1和MSV6.5（S1代）中，几乎一半的根尖细胞含有13条黑麦染色体，其余的含有12条黑麦染色体，两种植物的所有细胞都含有42条小麦染色体。在两种植物的花粉母细胞中观察到黑麦染色体的五种配对构型，包括5 II + 3 I、6 II + 1 I、6 II、5 II + 2 I和4 II + 4 I。这两种植物的后代，包括 S2、S3 和 S4 代植物，含有 42 条小麦染色体和 12 条黑麦染色体。因此，宽杂种体细胞中可能会发生染色体间易位和染色体不等分裂。体细胞中染色体分裂的不均等可导致异源多倍化早期染色体的消除。
Newly synthesized wheat–rye allopolyploids were investigated by genomic in situ hybridization, over the first, second, third and fourth allopolyploid generations. Inter and intra chromosome connections were observed in 12 root-tip cells of CA4.4.7 (S2generation), and translocations between wheat and rye chromosomes were also detected in five root-tip cells. In root-tip cells of CA4.4.7.5 and CA4.4.7.2.2 (S3and S4generation), the chromosome connections occurred again, a dissociative small rye segment was detected in seven cells of CA4.4.7.5. In plants MSV6.1 and MSV6.5 (S1generation), almost half of the root-tip cells contained 13 rye chromosomes and the rest held 12 rye chromosomes, and all the cells of the two plants contained 42 wheat chromosomes. Five pairing configurations of rye chromosomes, including 5 II + 3 I, 6 II + 1 I, 6 II, 5 II + 2 I and 4 II + 4 I, were observed in pollen mother cells of the two plants. The two plants’ progeny, including S2, S3, and S4generation plants, contained 42 wheat chromosomes and 12 rye chromosomes. Therefore, the inter chromosome translocation and unequal chromosome division could occur in somatic cells of wide hybrids. The unequal chromosome division in somatic cell could induce chromosome elimination at the early stages of allopolyploidization.