Three-dimensional structures self-assembled from DNA bricks.
Three-dimensional structures self-assembled from DNA bricks.
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DOI：
10.1126/science.1227268
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发表时间：
2012-11-30
期刊：
影响因子：
--
通讯作者：
Yin P
中科院分区：
文献类型：
--
作者：
Ke Y;Ong LL;Shih WM;Yin P
我们描述了一种简单而健壮的方法，使用短的合成DNA链来构建复杂的三维(3D)结构，我们称之为“DNA砖”。在一步退火反应中，具有数百个不同序列的砖自组装成指定的3D形状。每一块32个核苷酸的砖都是一个模块化组件；它与四个本地邻居结合，可以单独移除或添加。砖之间的每个8碱基对相互作用定义了一个尺寸为2.5纳米乘2.5纳米乘2.7纳米的体素，而主砖集合定义了尺寸为10乘10乘10体素的“分子画布”。通过从画布中选择砖块的子集，我们构建了一个由102个不同形状组成的面板，展示了复杂的表面特征以及复杂的内部洞穴和隧道。
We describe a simple and robust method to construct complex three-dimensional (3D) structures using short synthetic DNA strands that we call “DNA bricks”. In one-step annealing reactions, bricks with hundreds of distinct sequences self-assemble into prescribed 3D shapes. Each 32-nucleotide brick is a modular component; it binds to four local neighbors and can be removed or added independently. Each 8-base-pair interaction between bricks defines a voxel with dimensions 2.5 nanometers by 2.5 nanometers by 2.7 nanometers, and a master brick collection defines a “molecular canvas” with dimensions of 10 by 10 by 10 voxels. By selecting subsets of bricks from this canvas, we constructed a panel of 102 distinct shapes exhibiting sophisticated surface features as well as intricate interior cavities and tunnels.