Rapid marker-assisted development of advanced recombinant lines from barley starch mutants
Rapid marker-assisted development of advanced recombinant lines from barley starch mutants
复制标题
DOI：
10.1007/s11032-013-9930-0
复制
发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Greenland, Andy
中科院分区：
文献类型：
--
作者：
Howell, Phil;Leigh, Fiona;Greenland, Andy
突变体的比较表型分析往往受到其多样性和遗传背景不佳的阻碍。为了克服这个问题，一套重组春大麦品系开发的四个淀粉生物合成基因在一个共同的精英背景。采用前景选择、背景选择和混合家系筛选相结合的方法，使育种进展迅速。一个工具包的完美的共显性PCR检测开发的四个目标基因，基于其淀粉表型的致病单核苷酸多态性。这些用于回交和自交过程中的前景选择，并且可以应用于多达10株植物的批量。筛选批量意味着大量具有已知家族结构的个体被快速评估，育种工作被准确定位。这些标记也被用于质量控制在现场繁殖，并应容易转移到任何涉及这四个突变的杂交。在已知对目标淀粉等位基因杂合的BC 1后代中进行背景选择，识别出在基因组其余部分相对富集轮回亲本的个体。这些被进一步推进，并选择在很大程度上轮回亲本背景中携带靶淀粉突变的真正育种重组体。所得到的BC2F5预育种系组应能够对淀粉表型进行有意义的分析，并促进其转移到商业育种计划中。
The comparative phenotypic analysis of mutants is often hampered by their diverse and poorly characterised genetic backgrounds. To overcome this problem, a suite of recombinant spring barley lines was developed for four starch biosynthesis genes in a common elite background. Rapid breeding progress was made by combining foreground and background selection with the screening of bulked families. A toolkit of perfect co-dominant PCR assays was developed for the four target genes, based on the causative single nucleotide polymorphisms underlying their starch phenotypes. These were used for foreground selection during backcrossing and selfing, and may be applied to bulks of up to ten plants. Screening bulks meant that large numbers of individuals with known family structure were rapidly assessed and that breeding effort was accurately targeted. These markers were also used for quality control during field multiplication and should be readily transferable to any crosses involving these four mutations. Background selection amongst BC1 progeny known to be heterozygous for the target starch alleles identified individuals which were relatively enriched for the recurrent parent across the rest of the genome. These were further advanced and true-breeding recombinants were selected which carry the target starch mutations in a largely recurrent parent background. The resulting set of BC2F5 pre-breeding lines should enable meaningful analysis of the starch phenotypes and facilitate their transfer into commercial breeding programmes.