Renal Purge of Hemolymphatic Lipids Prevents the Accumulation of ROS-Induced Inflammatory Oxidized Lipids and Protects Drosophila from Tissue Damage
Renal Purge of Hemolymphatic Lipids Prevents the Accumulation of ROS-Induced Inflammatory Oxidized Lipids and Protects Drosophila from Tissue Damage
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DOI：
10.1016/j.immuni.2020.01.008
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发表时间：
2020-02-18
期刊：
影响因子：
32.4
通讯作者：
Lemaitre, Bruno
中科院分区：
文献类型：
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作者：
Li, Xiaoxue;Rommelaere, Samuel;Lemaitre, Bruno
动物需要复杂的代谢和生理适应来维持重要器官的功能，以应对环境压力和感染。在这里，我们发现感染或损伤的果蝇诱导血液淋巴脂排泄马氏小管，昆虫的肾脏。这种脂质清除是由应激诱导的脂质结合蛋白Materazzi介导的，该蛋白在马氏小管中富集。缺乏基质的果蝇血淋巴活性氧（ROS）浓度升高，脂质过氧化作用增加。这些果蝇也表现出马尔比氏小管功能障碍，易受感染和环境压力的影响。用抗氧化剂喂养果蝇可以挽救materazzi表型，这表明materazzi的主要作用是保护生物体免受应激诱导的ROS损伤。我们的研究结果表明，清除血淋巴脂是一种生理适应，可以在免疫和应激反应中保护宿主组织免受过多ROS的侵害，这一过程可能适用于其他生物体。
Animals require complex metabolic and physiological adaptations to maintain the function of vital organs in response to environmental stresses and infection. Here, we found that infection or injury in Drosophila induced the excretion of hemolymphatic lipids by Malpighian tubules, the insect kidney. This lipid purge was mediated by a stress-induced lipid binding protein, Materazzi, which was enriched in Malpighian tubules. Flies lacking materazzi had higher hemolymph concentrations of reactive oxygen species (ROS) and increased lipid peroxidation. These flies also displayed Malpighian tubule dysfunction and were susceptible to infections and environmental stress. Feeding flies with antioxidants rescued the materazzi phenotype, indicating that the main role of Materazzi is to protect the organism from damage caused by stress-induced ROS. Our findings suggest that purging hemolymphatic lipids presents a physiological adaptation to protect host tissues from excessive ROS during immune and stress responses, a process that is likely to apply to other organisms.