Titanium containing amorphous hydrogenated carbon films (a-C : H/Ti):: surface analysis and evaluation of cellular reactions using bone marrow cell cultures in vitro
Titanium containing amorphous hydrogenated carbon films (a-C : H/Ti):: surface analysis and evaluation of cellular reactions using bone marrow cell cultures in vitro
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DOI：
10.1016/s0142-9612(99)00135-0
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发表时间：
2000-03-01
期刊：
影响因子：
14
通讯作者：
Wintermantel, E
中科院分区：
文献类型：
--
作者：
Schroeder, A;Francz, G;Wintermantel, E
无定形氢化碳（a-C：H）涂层，也称为类金刚石碳（DLC），具有生物医学应用中保护涂层材料所需的许多特性。本研究的目的是通过结合a-C：H膜的硬度和惰性与钛的生物可接受性来评价骨相关植入物的新表面涂层。为此目的，不同量的钛被纳入a-C：H膜的组合射频（rf）和磁控溅射设置。空气暴露的a-C：H/Ti（a-C：H/Ti）薄膜的X射线光电子能谱（XPS）表明，薄膜由TiO 2和TiC嵌入并连接到a-C：H基体中组成。用原代成年大鼠骨髓细胞培养物（BMC）进行细胞培养试验，以确定对细胞数量以及对成骨细胞和破骨细胞分化的影响。通过向碳基质中添加钛，可以获得诸如增殖增加和破骨细胞样细胞活性降低的细胞反应，而这些反应在纯a-C：H膜和玻璃对照样品上未观察到。总之，a-C：H/Ti可通过支持骨细胞增殖同时减少破骨细胞样细胞活化而成为骨植入物的有价值的涂层。(C)2000爱思唯尔科技有限公司版权所有。
Amorphous hydrogenated carbon (a-C:H) coatings, also called diamond-like carbon (DLC), have many properties required for a protective coating material in biomedical applications. The purpose of this study is to evaluate a new surface coating for bone-related implants by combining the hardness and inertness of a-C : H films with the biological acceptance of titanium. For this purpose, different amounts of titanium were incorporated into a-C:H films by a combined radio frequency (rf) and magnetron sputtering set-up. The X-ray photoelectron spectroscopy (XPS) of air-exposed a-C:H/titanium (a-C:H/Ti) films revealed that the films were composed of TiO2 and TiC embedded in and connected to an a-C:H matrix. Cell culture tests using primary adult rat bone marrow cell cultures (BMC) were performed to determine effects on cell number and on osteoblast and osteoclast differentiation, By adding titanium to the carbon matrix, cellular reactions such as increased proliferation and reduced osteoclast-like cell activity could be obtained, while these reactions were not seen on pure a-C :H films and on glass control samples. In summary, a-C:H/Ti could be a valuable coating for bone implants, by supporting bone cell proliferation while reducing osteoclast-like cell activation. (C) 2000 Elsevier Science Ltd. All rights reserved.