Plasma cell development in synovial germinal centers in patients with rheumatoid and reactive arthritis.
Plasma cell development in synovial germinal centers in patients with rheumatoid and reactive arthritis.
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DOI：
10.4049/jimmunol.162.5.3053
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发表时间：
1999-03
影响因子：
4.4
通讯作者：
Hye-Jung Kim;V. Krenn;G. Steinhauser;C. Berek
中科院分区：
文献类型：
--
作者：
Hye-Jung Kim;V. Krenn;G. Steinhauser;C. Berek
在类风湿和反应性关节炎患者的滑膜组织中，在巨噬细胞、T和B细胞的炎性浸润周围发现浆细胞。这种特征性排列表明，在滑膜组织中，CD 20 + B细胞分化为浆细胞。为了检测克隆关系，我们使用显微操作从单个浸润细胞中分别分离CD 20 + B细胞和浆细胞。提取DNA，并从两个群体中确定VH/VL基因库。数据表明，在发炎的滑膜组织中，活化的B细胞是克隆扩增的。在滤泡树突状细胞网络的增殖过程中，V基因变体通过超突变机制产生。令人惊讶的是，我们没有发现相同的重排之间的CD 20 + B细胞和浆细胞。然而，在单个浸润组织中发现克隆相关的浆细胞表明这些细胞在滑膜组织中经历了终末分化。这些结果表明，B细胞在滑膜组织中的分化是一个动态的过程。尽管CD 20 + B细胞可以快速更新，浆细胞可以很好地存活，因此在滑膜组织中积累。对从滑膜组织中回收的单个B细胞的分析为确定参与局部免疫反应的那些细胞的特异性开辟了一条新途径。这将为慢性炎症性疾病如类风湿性关节炎或反应性关节炎的发病机制提供新的见解。
Plasma cells are found surrounding the inflammatory infiltrates of macrophages, T, and B cells in the synovial tissue of patients with rheumatoid and reactive arthritis. This characteristic arrangement suggests that in the synovial tissue CD20+ B cells differentiate into plasma cells. To examine clonal relationships, we have used micromanipulation to separately isolate CD20+ B cells and plasma cells from single infiltrates. DNA was extracted, and from both populations the VH/VL gene repertoires was determined. The data show that in the inflamed synovial tissue activated B cells are clonally expanded. During proliferation in the network of follicular dendritic cells, V gene variants are generated by the hypermutation mechanism. Surprisingly, we do not find identical rearrangements between CD20+ B cells and plasma cells. Nevertheless, the finding of clonally related plasma cells within single infiltrates suggests that these cells underwent terminal differentiation in the synovial tissue. These results indicate that B cell differentiation in the synovial tissue is a dynamic process. Whereas CD20+ B cells may turnover rapidly, plasma cells may well be long lived and thus accumulate in the synovial tissue. The analysis of individual B cells recovered from synovial tissue opens a new way to determine the specificity of those cells that take part in the local immune reaction. This will provide new insights into the pathogenesis of chronic inflammatory diseases like rheumatoid or reactive arthritis.