A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays
A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays
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DOI：
10.1063/1.2830286
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Lam, K. S.
中科院分区：
文献类型：
--
作者：
Fei, Y. Y.;Landry, J. P.;Lam, K. S.
我们描述了一种新的扫描光学显微镜的基础上偏振调制调零椭圆偏振。新的显微镜采用扫描镜和样品平移的组合，从而能够高通量无标记检测生物分子微阵列与超过10 000蛋白质或小分子目标。为了说明，我们展示了用这种显微镜获得的功能化载玻片上的2760点蛋白质微阵列的图像。新的扫描显微镜也能够确定，在平行，实时结合动力学的多个分子物种在水性条件下。(c)2008年美国物理学会。
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions. (c) 2008 American Institute of Physics.