The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus.
The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus.
复制标题
海胆 S. purpuratus 中两个连锁肌动蛋白基因的染色体排列。
DOI：
10.1093/nar/9.3.591
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发表时间：
1981
影响因子：
14.9
通讯作者：
Keller,EB
中科院分区：
文献类型：
--
作者：
Schuler,MA;Keller,EB
从重组的Charon 4噬菌体基因组文库中分离到了紫海胆的4个不同的肌动蛋白基因。这四个基因在它们的许多限制位点上彼此有很大的不同。这四个基因中有两个紧密相连；它们存在于克隆的DNA的同一片段中。这个片段已经被广泛定位，DNA的一些部分已经被测序。这两个相连的基因指向相同的方向，被7.5kb的DNA分开。在CAG之后有一个内含子，编码肌动蛋白氨基酸序列中第121位的谷氨酰胺残基。这是在肌动蛋白基因中发现的第五个不同的内含子位置，表明原始肌动蛋白基因至少有6个外显子和5个内含子。肌动蛋白基因形成了一个独特的家族，在这个家族中，大多数内含子显然已经从基因中精确地切除了。
Four distinct actin genes of the sea urchinStrongylocentrotus purpuratushave been isolated from a recombinant Charon 4 phage library of genomic DNA. The four genes differ considerably from each other in many of their restriction sites. Two of the four genes are closely linked; they are present in the same fragment of cloned DNA. This fragment has been extensively mapped, and some parts of the DNA have been sequenced. The two linked genes are oriented in the same direction, separated by 7.5 kb of DNA. One has an intron following the CAG that codes for the glutamine residue at position 121 in the amino acid sequence of actin. This represents the fifth distinct site at which introns have been found in actin genes, suggesting that the primordial actin gene had at least 6 exons and 5 introns. The actin genes form a distinctive family in which most introns have apparently been precisely excised from the genes.