Differential expression of a novel gene EaF82a in green and yellow sectors of variegated Epipremnum aureum leaves is related to uneven distribution of auxin.
Differential expression of a novel gene EaF82a in green and yellow sectors of variegated Epipremnum aureum leaves is related to uneven distribution of auxin.
复制标题
一种新基因 EaF82a 在杂色 Epipremnum aureum 叶子的绿色和黄色部分中的差异表达与生长素的不均匀分布有关。
DOI：
10.1111/ppl.12219
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发表时间：
2014
影响因子：
6.4
通讯作者：
Xie,Jiahua
中科院分区：
文献类型：
--
作者：
Hung,Chiu-Yueh;Umstead,MakendraL;Chen,Jianjun;Holliday,BronwynM;Kittur,FarooqahmedS;Henny,RichardJ;Burkey,KentO;Xie,Jiahua
EaF82, a gene identified in previous studies of the variegated plantEpipremnum aureum, exhibited a unique expression pattern with greater transcript abundance in yellow sectors than green sectors of variegated leaves, but lower abundance in regenerated pale yellow plants than in green plants derived from leaf tissue culture. Studies of its full‐length cDNA and promoter region revealed two members with only theEaF82aexpressed. Immunoblotting confirmed thatEaF82aencodes a 12 kDa protein and its accumulation consistent with its gene expression patterns in different color tissues. Transient expression of EaF82a‐sGFP fusion proteins in protoplasts showed that EaF82a seems to be present in the cytosol as unidentified spots. Sequence motif search reveals a potential auxin responsive element in promoter region. Using transgenic Arabidopsis seedlings carryingEaF82apromoter driving the bacterialuidA (GUS) gene, an increased GUS activity was observed when IAA (indole‐3‐acetic acid) concentration was elevated. InE. aureum,EaF82ais more abundant at the site where axillary buds emerge and at the lower side of bending nodes where more IAA accumulates relative to the upper side. The measurement of endogenous IAA levels in different color tissues revealed the same pattern of IAA distribution as that ofEaF82aexpression, further supporting thatEaF82ais an IAA responsive gene.EaF82aexpression in etiolated transgenic Arabidopsis seedlings responded to IAA under the influence of light suggesting a microenvironment of uneven light condition affects theEaF82atranscript levels and protein accumulation in variegated leaves.
