A novel glutathione-S transferase immunosensor based on horseradish peroxidase and double-layer gold nanoparticles
A novel glutathione-S transferase immunosensor based on horseradish peroxidase and double-layer gold nanoparticles
复制标题
基于辣根过氧化物酶和双层金纳米粒子的新型谷胱甘肽-S转移酶免疫传感器
DOI：
10.1007/s10544-016-0075-x
复制
发表时间：
2016-06-01
影响因子：
2.8
通讯作者：
Pang, Guangchang
中科院分区：
文献类型：
--
作者：
Lu, Dingqiang;Lu, Fuping;Pang, Guangchang
GSTs, a biotransformation enzyme group, can perform metabolism, drug transfer and detoxification functions. Rapid detection of the GSTs with more sensitive approaches is of great importance. In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode (DGN-EIE) immobilized with Glutathione S-Transferase (GST) antibody derived from Balb/c mice was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, transmission electron microscope (TEM) was used to characterize the nanogold solution. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine the GST in PBS. The results showed that the response current had a good linear correlation with the GST concentration ranged from 0.1–104pg/mL. The lowest detection limit was found at 0.03 pg/mL(S/N= 3). The linear equation was deduced as △I/% = 7.386lgC + 22.36 (R2= 0.998). Moreover, it was validated with high sensitivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the GST.
