Genetic control of resistance to the sterol 14α-demethylase inhibitor fungicide prochloraz in the cereal eyespot pathogen Tapesia yallundae
Genetic control of resistance to the sterol 14α-demethylase inhibitor fungicide prochloraz in the cereal eyespot pathogen Tapesia yallundae
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DOI：
10.1128/aem.66.11.4599-4604.2000
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发表时间：
2000-11-01
影响因子：
4.4
通讯作者：
Lucas, JA
中科院分区：
文献类型：
--
作者：
Dyer, PS;Hansen, J;Lucas, JA
有性杂交被用来确定谷物眼斑病原体Tapestryallundae对甾醇14 α-脱甲基酶抑制剂杀菌剂咪鲜胺抗性的遗传基础。敏感亲本菌株之间的三种不同杂交（22-432和22-433 [抑制生长50%所需的浓度（IG（50））均小于或等于0.03 mg/L]）和来自法国和新西兰的具有不同抗性水平的田间分离物（PR 11 [IG（50）= 0.5 mg/L]、PR 1 [IG（50）= 1.0 mg/L]和11-3-18 [IG（50）= 2.4 mg/L]）产生的后代显示双峰分布，具有偶数个敏感和抗性后代。这表明在每个杂交中分离了一个单一的抗性主基因，这一点通过回交、F（1）后代间杂交和敏感亲本间对照杂交得到了证实。然而，也有证据表明，额外的定量遗传成分导致了耐药性较强的分离株的IG（50）增加。在分离物PR 11和分离物11-3-18产生的F（1）后代之间进行进一步杂交，这也产生了完全抗咪鲜胺的后代。这表明，抗性基因在这两个分离物中是等位的，抗性由同一位点（或紧密连锁的位点）的基因赋予，尽管分离物（PR 11和11-3-18）来自不同的大陆。
Sexual crosses were used to determine the genetic basis of resistance to the sterol 14 alpha -demethylase inhibitor fungicide prochloraz in the cereal eyespot pathogen Tapesia yallundae. Three different crosses between sensitive parental strains (22-432 and 22-433 [the concentration required to inhibit growth by 50% (IG(50)) for each was less than or equal to0.03 mg/liter]) and field isolates from France and New Zealand with differing levels of resistance (PR11 [IG(50) = 0.5 mg/liter], PR1 [IG(50) = 1.0 mg/liter], and 11-3-18 [IG(50) = 2.4 mg/liter]) yielded progeny showing a bimodal distribution, with an even number of sensitive and resistant progeny. This indicated the segregation of a single major gene for resistance in each cross, which was confirmed by the use of backcrosses, crosses between F(1) progeny, and control crosses between sensitive parents. However, there was also evidence of additional quantitative genetic components responsible for the increased IG(50)s of the more resistant isolates. A further cross was made between isolate PR11 and an F(1) progeny arising from isolate 11-3-18, and this also yielded progeny which were entirely prochloraz resistant. This suggested that resistance genes were allelic in these two isolates, with resistance conferred by a gene at the same locus (or closely linked loci), despite the fact that the isolates (PR11 and 11-3-18) originated from different continents.