IMMUNOHISTOCHEMICAL LOCALIZATION OF COMPONENTS OF THE IMMUNE BARRIER IN THE OLFACTORY MUCOSAE OF SALAMANDERS AND RATS
IMMUNOHISTOCHEMICAL LOCALIZATION OF COMPONENTS OF THE IMMUNE BARRIER IN THE OLFACTORY MUCOSAE OF SALAMANDERS AND RATS
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DOI：
10.1002/ar.1092310310
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发表时间：
1991-11-01
影响因子：
--
通讯作者：
GETCHELL, TV
中科院分区：
文献类型：
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作者：
GETCHELL, ML;GETCHELL, TV
应用免疫组织化学技术研究了分泌免疫系统的组成成分，包括分泌免疫球蛋白、分泌片段和J链，以及其它免疫球蛋白和非特异性防御因子在蝾螈和大鼠嗅粘膜中的细胞分布。和嗅粘膜未成熟区域的支持细胞中。乳铁蛋白和溶菌酶也存在于鲍曼氏腺中，在嗅粘膜的未成熟区域的支持细胞中，以及在固有层中的血细胞中。嗅神经切片导致分泌型免疫球蛋白免疫反应性B淋巴细胞数量增加，IgM、分泌片和乳铁蛋白分布改变。在大鼠中，分泌型免疫球蛋白A和J链定位于鲍曼氏腺的导管和腺泡细胞以及固有层中的B淋巴细胞。只有在先前有病毒感染的大鼠中才能在Bowman腺中证明分泌片。位于固有层中的其他防御因子包括结缔组织基质和B淋巴细胞中的IgG、IgD免疫反应性B淋巴细胞和被鉴定为粘膜肥大细胞的IgE免疫反应性细胞。乳铁蛋白和溶菌酶存在于鲍曼氏腺的浆液性腺泡细胞和血细胞中。这些结果表明，嗅觉粘膜受到分泌免疫系统以及其他免疫球蛋白、乳铁蛋白和溶菌酶的保护，免受病原体的侵袭。
Immunohistochemical techniques were used to investigate the cellular distribution of components of the secretory immune system, including secretory immunoglobulin, secretory piece, and J chain, as well as other immunoglobulins and nonspecific defense factors in the olfactory mucosae of salamanders and rats.In the salamander, secretory immunoglobulin M, and J chain were localized in duct and acinar cells of Bowman's glands, in B lymphocytes, and in sustentacular cells in immature regions of the olfactory mucosa. Lactoferrin and lysozyme were also present in Bowman's glands, in sustentacular cells in immature regions of the olfactory mucosa, and in blood cells in the lamina propria. Olfactory nerve section resulted in the presence of increased numbers of secretory immunoglobulin-immunoreactive B lymphocytes and in an altered distribution of IgM, secretory piece, and lactoferrin.In the rat, secretory immunoglobulin A and J chain were localized in duct and acinar cells of Bowman's glands and in B lymphocytes in the lamina propria. Secretory piece could be demonstrated in Bowman's glands only in rats that had a prior viral infection. Other defense factors, localized in the lamina propria, included IgG in the connective tissue stroma and in B lymphocytes, IgD-immunoreactive B lymphocytes, and IgE-immunoreactive cells that were identified as mucosal mast cells. Lactoferrin and lysozyme were present in serous acinar cells of Bowman's glands and in blood cells.These results demonstrate that the olfactory mucosa is protected from pathogenic invasion by the secretory immune system as well as other immunoglobulins, lactoferrin, and lysozyme.