Post-stress metabolism involves umbelliferone production in anthocyanin-producing and non-producing cells of Glehnia littoralis suspension cultures
Post-stress metabolism involves umbelliferone production in anthocyanin-producing and non-producing cells of Glehnia littoralis suspension cultures
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DOI：
10.1016/j.jplph.2004.11.014
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发表时间：
2005-06-01
影响因子：
4.3
通讯作者：
Watanabe, M
中科院分区：
文献类型：
--
作者：
Ishikawa, A;Kitamura, Y;Watanabe, M
研究了酵母膏对紫皂角和非花青素细胞悬浮培养48h苯丙氨酸解氨酶(PAL，EC 4.1.3.5)和查尔酮合成酶(CHS，EC 2.3.1.74)转录物积累、PAL和CHS酶活性以及呋喃香豆素和花青素代谢产物的影响。在此过程中，在紫罗兰的培养液和白色细胞中都检测到了以前没有检测到的伞形酮。在白细胞中，PAL转录物的积累和PAL活性的增加与伞形酮的水平很好地一致，其次是Bergapten的诱导。在紫罗兰细胞中，PAL和CHS转录本的积累，PAL和CHS酶活性以及花青素水平的增加，都是导致PAL和CHS转录本的积累。其中在未经处理的细胞中高表达的基因被暂时抑制。然而，对PAL转录物和PAL活性的抑制不如对CHS转录物积累和CHS活性的抑制，这可能是诱导后不久伞形酮产量急剧增加的一个因素。(C)2005年爱思唯尔股份有限公司。版权所有。
The effects of yeast extract on the accumulation of transcripts of phenylalanine ammonia-lyase (PAL, EC 4.1.3.5) and chalcone synthase (CHS, EC 2.3.1.74), PAL and CHS enzyme activity and furanocoumarin and anthocyanin metabolites over a 48 h period were studied in anthocyanin-producing (Violet) and non-producing (White) cell suspension cultures of Glehnia littoralis. In the course of this period, umbelliferone, which had not been detected earlier, was detected in the culture medium of the Violet as well as White cells. In White cells, the PAL transcript accumulation and an increase in PAL activity were in good agreement with the level of umbelliferone, and was followed by the induction of bergapten. In the case of the Violet cells, the accumulation of PAL and CHS transcripts, and the increases in PAL and CHS enzyme activity as well as the anthocyanin level, all. of which were highly expressed in non-treated cells, were temporarily suppressed. However, the suppression of the PAL transcript and PAL activity was not as great as that of the CHS transcript accumulation and CHS activity, in which a sharp transient increase of umbelliferone production soon after elicitation appears to be a factor. (c) 2005 Elsevier GmbH. All rights reserved.