Whole and Strip Nitrocellulose Membrane as well as New Line Immunoblotting of Antigen Using the Chemiluminescence Technique
Whole and Strip Nitrocellulose Membrane as well as New Line Immunoblotting of Antigen Using the Chemiluminescence Technique
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DOI：
10.1007/978-1-59745-542-8_42
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发表时间：
2009-01-01
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影响因子：
--
通讯作者：
Scofield, R. Hal
中科院分区：
文献类型：
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作者：
Dorri, Yaser;Khalili, Ali W.;Scofield, R. Hal
科学界有许多技术可供研究人员用来检测特定抗原。一种这样的技术提供了许多优于典型免疫化学染色的优点，即化学发光。几个世纪以来，人们已经知道化合物一旦暴露在阳光下就会发射可见辐射，这是化学发光的主要原理。在此，我们介绍了三种不同的化学发光技术，广泛应用于免疫检测抗原：（a）全膜化学发光检测，（B）带膜化学发光检测，（c）新线印迹化学发光。
There are a number of techniques in the scientific world that researchers use to detect specific antigens. One such technique that has provided many advantages over typical immunochemical staining is chemiluminescence. The emission of visible radiation by compounds once exposed to sunlight has been known for centuries and currently is the main principle for chemiluminescence. Here, we introduce three different chemiluminescence techniques that are widely used in immunodetection of antigens: (a) whole membrane chemiluminescence detection, (b) strip membrane chemiluminescence detection, and (c) new line blotting chemiluminescence.