Brown adipose tissue mitochondrial subpopulations show different morphological and thermogenic characteristics
Brown adipose tissue mitochondrial subpopulations show different morphological and thermogenic characteristics
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DOI：
10.1016/j.mito.2004.09.003
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发表时间：
2005-02-01
期刊：
影响因子：
4.4
通讯作者：
Gianotti, M
中科院分区：
文献类型：
--
作者：
Justo, R;Oliver, J;Gianotti, M
大鼠棕色脂肪组织线粒体亚群-在1000,3000和8000g差速离心分离，给予重，中，轻线粒体-被表征。因此，非线粒体亚细胞成分污染、形态特征、呼吸链和抗氧化酶活性、解偶联蛋白I和线粒体蛋白含量、线粒体DNA水平和线粒体完整性均被测量。结果表明，线粒体组分在形态、产热和抗氧化性能上存在重要差异。所有研究参数在重线粒体中都较高，这表明线粒体分化状态更强。(C) 2004 Elsevier B.V.与线粒体研究学会。版权所有。
Rat brown adipose tissue mitochondrial subpopulations-isolated by differential centrifugation at 1000, 3000 and 8000g, giving the heavy, medium and light mitochondria-were characterized. Thus, contamination by non-mitochondrial subcellular components, morphological features, respiratory chain and antioxidant enzyme activities, both uncoupling protein I and mitochondrial protein content, mitochondrial DNA levels and mitochondrial integrity were measured. Results indicate that mitochondrial fractions showed important differences in the morphological, thermogenic and antioxidant properties. All the parameters studied were always higher in heavy mitochondria, which is indicative of a greater mitochondrial differentiation state. (C) 2004 Elsevier B.V. and Mitochondria Research Society. All rights reserved.